Table. 1.

Revised classification of general chemistry test methods of the Korean Association of External Quality Assessment Service

Test items Current classification Revised classification
Glucose 1. Hexokinase 1. Hexokinase
2. Glucose oxidase 2. Glucose oxidase
3. Glucose dehydrogenase
4. Other method
Total protein 1. Biuret method 1. Biuret method
2. Other method
Albumin 1. Dye binding–BCG 1. Dye binding–BCG
2. Dye binding–BCP 2. Dye binding–BCP
3. Other method
Blood urea nitrogen 1. Urease with GLDH (coupled enzymes) 1. Urease with GLDH
2. Enzymatic UV test (urease–GLDH) 2. Urease with conductivity
3. Urease quinolinium dye (Vitros) 3. Urease with colorimetry
4. Conductivity rate method 4. Dry chemistry
5. Urease–bromocresol green
6. Urease–indophenol (Berthelot)
7. Ammonium ISE, urease
8. Other method
Aspartate transaminase 1. UV without P5P 1. MDH without P5P
2. Enzymatic, colorimetric (Vitros) 2. MDH with P5P
3. UV with P5P 3. Dry chemistry
4. Dinitrophenyl hydrazine
5. Other method
Alanine transaminase 1. UV without P5P 1. LDH without P5P
2. Enzymatic, colorimetric (Vitros) 2. LDH with P5P
3. UV with P5P 3. Dry chemistry
4. Dinitrophenyl hydrazine
5. Other method
Total bilirubin 1. Vanadate oxidation 1. Diazo-dimethyl sulfoxide with blank
2. Diazonium salt/diazonium ion with blank 2. Diazo-dimethyl sulfoxide without blank
3. Oxidation 3. Diazo-caffeine, sodium benzoate with blank
4. Diazo-dimethyl sulfoxide with blank 4. Diazo-caffeine, sodium benzoate without blank
5. Evelyn-Malloy method without blank 5. Diazo-dyphylline with blank
6. Colorimetric test (DCA/DPD) 6. Diazo-dyphylline without blank
7. Enzymatic bilirubin oxidase 7. Diazonium salt-surfactant with blank
8. Evelyn-Malloy method with blank 8. Diazonium salt-surfactant without blank
9. Diazonium salt/diazonium ion without blank 9. Diazonium salt-dyphylline with blank
10. Diazonium salt-dyphylline (Vitros) 10. Diazonium salt-dyphylline without blank
11. Jendrassik-Grof method with blank 11. Copper ion oxidation
Total bilirubin 12. Benzenesulfonic acid diazo reaction 12. Vanadate oxidation
13. Jendrassik-Grof method without blank 13. Enzymatic oxidation
14. Other method
Direct bilirubin 1. Vanadate oxidation 1. Diazo-sulfanilic acid with blank
2. Diazonium salt/diazonium ion with blank 2. Diazo-sulfanilic acid without blank
3. Colorimetric test (DCA/DPD) 3. Diazonium salt with blank
4. Enzymatic bilirubin oxidase 4. Diazonium salt without blank
5. Oxidation 5. Copper ion oxidation
6. Evelyn-Malloy method with blank 6. Vanadate oxidation
7. Evelyn-Malloy method without blank 7. Enzymatic oxidation
8. Diazonium salt/diazonium ion without blank
9. Oxidation direct bilirubin
10. Other method (modified Jendrassik-Groff using surfactant as an acc)
11. Jendrassik-Grof method with blank
12. Benzenesulfonic acid diazo reaction
13. Diazo-dimethyl sulfoxide with blank
14. Other method
Alkaline phosphatase 1. PNPP, AMP buffer 1. PNPP, AMP buffer
2. PNPP, EAE buffer 2. PNPP, EAE buffer
3. PNPP, DEA buffer 3. PNPP, DEA buffer
4. 4-Aminoantipyrine
5. Other method
Total cholesterol 1. Enzymatic method 1. Cholesterol esterase
2. Other method
Triglyceride 1. Enzymatic (glycerol phosphate oxidase) without glycerol blank 1. Enzymatic with free glycerol elimination
2. Enzymatic (glycerol phosphate oxidase) with glycerol blank 2. Enzymatic without free glycerol elimination
3. Enzymatic (others) with glycerol blank
4. Enzymatic (others) without glycerol blank
5. Other method
LDL cholesterol 1. Sekisui LDL direct method 1. Enzymatic with detergent
2. Direct method (other company) 2. Enzymatic with polyethylene glycol
3. Roche LDL direct method 3. Enzymatic with others
4. Beckman AU LDL direct method
5. Kyowa LDL direct method
6. Wako LDL direct method
7. Siemens Advia direct LDL method
LDL cholesterol 8. Siemens LDL direct method
9. Randox LDL direct method
10. Denka LDL direct method
11. Other method
HDL cholesterol 1. Direct method (other company) 1. Enzymatic with polyethylene glycol
2. Sekisui HDL direct method 2. Enzymatic with detergent
3. Roche HDL direct method 3. Enzymatic with immunoinhibition
4. Beckman AU HDL direct method 4. Enzymatic with polyanion precipitation
5. Kyowa HDL direct method 5. Enzymatic with electrostatic
6. Wako HDL direct method 6. Enzymatic with others
7. Siemens Advia direct HDL method
8. Siemens HDL direct method
9. Denka HDL direct method
10. Randox HDL direct method
11. Other method
Amylase 1. Hydrolysis of 4,6-ethylidene-4-nitrophenyl (4-NP)-maltohetaose (G7) sub. 1. Hydrolysis of 4,6-ethylidene-4-nitrophenyl-maltoheptaose
2. Hydrolysis of 2-chloro-p-nitrophenyl-α-D-maltotrioside (CNP-G3) 2. Hydrolysis of 2-chloro-4-nitrophenyl-α-D-maltotriose
3. Hydrolysis of 4-nitrophenyl (4-NP)-maltohetaose (G7) substrate 3. Hydrolysis of 4-nitrophenylbenzyl-α-maltopentaose
4. Other method 4. Hydrolysis of 2-chloro-4-nirophenyl-galactopyranosylmaltoside
5. Hydrolysis of 2-chloro-4-nitrophenyl-azido-deoxy-maltopentaoside
6. Dry chemistry
Lipase 1. Hydrolysis of 1,2-O-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) 1. Hydrolysis of 1,2-O-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester
2. Spectrophotometric method with triglyceride 2. Hydrolysis of 1,2-diglyceride with quinone dye
3. Hydrolysis of 1-oleoyl-2,3-diacetyl glycerol 3. Hydrolysis of 1-oleoyl-2,3-diacetyl glycerol
4. Other enzymatic method
5. Turbidimetric method with triglyceride
6. Other method
Gamma-glutamyl transferase 1. G-glutamyl-carboxy-nitroanilide (IFCC) 1. Gamma-glutamyl-carboxy-nitroanilide
2. G-glutamyl-carboxy-nitroanilide (JSCC) 2. Gamma-glutamyl-p-nitroanilide
3. G-glutamyl-p-nitroanilide 3. Gamma-glutamyl-p-nitroanilide (dry chemistry)
4. G-glutamyl-p-nitroanilide (dry chemistry)
5. Other method
Lactate dehydrogenase 1. Lactate to pyruvate 1. Lactate to pyruvate
2. Pyruvate to lactate 2. Pyruvate to lactate
3. Pyruvate to lactate (dry chemistry) 3. Pyruvate to lactate (dry chemistry)
4. Dinitrophenylhydrazine
5. Tetrazolium
6. Other method
Creatine kinase 1. Reverse (CrP→Cr) reaction with cofactor 1. CrP to Cr with activator
2. Reverse (CrP→Cr) reaction 2. CrP to Cr
3. Other method 3. Dry chemistry
Uric acid 1. Uricase 1. Uricase
2. Uricase (Vitros) 2. Uricase (dry chemistry)
3. Other method
Calcium 1. Cresolphthalein complexone 1. Colorimetry o-cresolphthalein complexone
2. Arsenazo III dye 2. Colorimetry_arsenazo III
3. NM-BAPTA 3. Colorimetry_MXB
4. MXB method (MXB) 4. Colorimetry_CPN III
5. ISE, diluted 5. NM-BAPTA
6. CPN III
7. Other method
Phosphorus 1. Phosphomolybdate, UV 1. Phosphomolybdate
2. Phosphomolybdate with any reduction method 2. Molybden blue colorimetry
3. Enzymatic method 3. Enzymatic
4. Other method
Magnesium 1. Colorimetric (dye-xylidyl blue/Magon) 1. Colorimetry_xylidyl blue
2. Colorimetrc test-xylidyl blue 2. Colorimetric_calmagite
3. Colorimetric–Vitros Chemistry Systems 3. Colorimetric_CPN III
4. Calmagite 4. Dry chemistry
5. CPN III
6. Arsenazo
7. Colorimetric (dye-methylthymol blue)
8. Enzymatic-glycerol kinase
9. Enzymatic-isocitrate dehydrogenase
10. Other method
Sodium, potassium, chloride 1. ISE, undiluted (direct) 1. ISE, undiluted
2. ISE, diluted (indirect) 2. ISE, diluted
3. Colorimetric ionophore
4. Other method
Iron 1. Ferrachrome/ferrozine without deproteinization 1. Ferrozine without deproteinization
2. Bathophenanthroline without deproteinization 2. Bathophenanthroline without deproteinization
3. TPZ: TPZ without prior protein removal 3. TPZ without deproteinization
4. Nitroso-PSAP without deproteinization 4. Nitroso-PSAP without deproteinization
5. Pyridyl azo dye ascorbate without deproteinization 5. Pyridyl azo dye ascorbate without deproteinization
6. Ferene without deproteinization 6. Ferene with deproteinization
7. Ferrachrome/ferrozine with deproteinization
8. Ferene with deproteinization
9. Other method
Total iron binding capacity 1. Bathophenanthroline 1. Bathophenanthroline
2. Ferrachrome/ferrozine 2. Ferrozine
3. Nitroso-PSAP 3. Nitroso-PSAP
4. Chromazural B 4. Chromazural B
5. Ferene
6. Other method

Abbreviations: KEQAS, Korean Association of External Quality Assessment Service; BCG, bromocresol green; BCP, bromocresol purple; GLDH, glutamate dehydrogenase; UV, ultraviolet; ISE, ion-selective electrode; P5P, pyridoxal 5'-phosphate; MDH, malate dehydrogenase; LDH, lactate dehydrogenase; DCA, dichloroacetic acid; DPD, N,N-diethyl-p-phenylenediamine; PNPP, p-nitrophenyl phosphate; AMP, alkaline phosphatase; EAE, ethylaminoethanol; DEA, diethanolamine; LDL, low-density lipoprotein; HDL, high density lipoprotein; IFCC, International Federation of Clinical Chemistry and Laboratory Medicine; JSCC, Japan Society of Clinical Chemistry; CrP, creatine phosphate; Cr, creatine; NM-BAPTA, 5-nitro-5'-methyl-BAPTA; MXB, methylxylenol blue; CPN III, chlorophosphonazo III; TPZ, tripyridyltriazine; Nitroso-PSAP, 2-nitroso-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol.

Lab Med Qual Assur 2021;43:9~18 https://doi.org/10.15263/jlmqa.2021.43.1.9
© Lab Med Qual Assur