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Evaluation of the Vacuum Blood Collection Tube, V-Tube, a Domestic Product for Clinical Chemistry and Thyroid Hormone Assays
J Lab Med Qual Assur 2018;40:211-222
Published online December 31, 2018
© 2018 Korean Association of External Quality Assessment Service.

Sung Jin Jo1, Yeong Sic Kim1, Sang-Guk Lee2, Jehoon Lee1

1Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea,
2Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea
Correspondence to: Jehoon Lee Department of Laboratory Medicine, Yeouido St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, 10 63(yuksam)-ro, Yeongdeungpo-gu, Seoul 07345, Korea Tel: +82-2-3779-1297 Fax: +82-2-3779-2285 E-mail: lyejh@catholic.ac.kr
Received July 17, 2018; Revised September 18, 2018; Accepted October 2, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract

Background:

It is known that the blood collection tube used can cause fluctuations in laboratory test results. We compared test results obtained when blood was collected in V-tube (AB Medical, Korea), BD Vacutainer Tubes (BD, USA), and Greiner Vacuette Tubes (Greiner, USA) in clinical chemistry and thyroid hormone assays.

Methods:

One hundred volunteers from three hospitals were recruited and the peripheral blood samples were collected in each of the three serum separation tubes (SSTs). These samples were used for 28 routine clinical chemistry assays and three thyroid hormone assays. The results were analyzed by the Student paired t-test and the Bland-Altman plot. For stability tests, the initial results were compared with the day 1 (24±2 hours), day 3 (72±2 hours), and day 7 (168±2 hours) results, respectively.

Results:

The difference in the test results obtained from the samples in each tube (V-Tube vs. BD-Tube, V-Tube vs. Greiner-Tube, and BD-Tube vs. Greiner-Tube) were satisfied with the Clinical Laboratory Improvement Amendments of 1988 allowable difference ranges. Except for four analytes (low-density lipoprotein cholesterol, magnesium, potassium, and thyroid-stimulating hormone), all analytes were within the allowable critical difference range based on biological variability. The paired t-test revealed significant differences between the results of nine assays for samples in V-Tube vs. BD-Tube and seven assays for samples in V-Tube vs. Greiner-Tube, but each set of results showed good correlations. The test results on different days showed a significant difference in several assays, but they were within the allowable difference range.

Conclusions:

The assay results for blood samples collected in SST V-Tubes were comparable to those obtained when blood was collected in BD Tubes and Greiner Tubes, and the blood collected in V-Tubes also showed excellent results in the stability tests.

Keywords : Serum separation tube, Chemistry, V-Tube
꽌 濡

쟻젅븳 삁븸 梨꾩랬 寃궗쓽 吏꾪뻾 젙솗븳 寃궗寃곌낵瑜 留뚮뱶뒗 寃궗 쟾 슂냼씠떎. 吏꾨떒寃궗빆紐⑹뿉꽌 梨꾪삁愿쓽 뭹吏덇낵 삱諛붾Ⅸ 痍④툒 寃궗寃곌낵뿉 겙 쁺뼢쓣 以 닔 엳쓬뿉룄 遺덇뎄븯怨 以묒슂꽦뿉 鍮꾪븯뿬 留롮 愿떖쓣 諛쏆 紐삵븯怨 엳떎[1]. 寃궗떎뿉꽌 떆뻾릺뒗 留롮 寃궗뱾 梨꾪삁愿쓣 씠슜븯硫 洹 以 吏꾧났梨꾪삁愿 젙빐吏 슜웾쓣 떞쓣 닔 엳뒗 湲곕뒫怨 뜑遺덉뼱 寃泥댁쓽 蹂닿 諛 痍④툒옄쓽 蹂댄샇뿉 옣젏씠 엳뼱꽌 1940뀈 媛쒕컻맂 씠옒濡 꼸由 궗슜릺怨 엳떎[2]. 珥덉갹湲곗뿉뒗 쑀由 옱吏덉쓽 梨꾪삁愿씠 궗슜릺뿀怨 궛뾽냼옱쓽 諛쒖쟾怨 痍④툒긽쓽 렪由ы븿 븣臾몄뿉 쁽옱뒗 遺遺 슜룄뿉꽌 뵆씪뒪떛 옱吏덉쓽 梨꾪삁愿씠 궗슜맂떎. 吏꾧났梨꾪삁愿 슜룄뿉 뵲씪 빆쓳怨좎젣 clot activator 諛 polymer gel 벑쓣 룷븿븯뿬 떆뙋릺硫 씠윭븳 臾쇱쭏뱾 삉븳 寃궗寃곌낵뿉 쁺뼢쓣 誘몄튌 닔 엳뒗 슂냼씠떎[3]. 쁽옱 슦由щ굹씪뿉꽌뒗 닔엯젣뭹씤 Becton Dickinson궗쓽 Vacutainer 吏꾧났梨꾪삁愿(BD, Flanklin Lakes, NJ, USA)怨 Greiner궗쓽 Vacuette 吏꾧났梨꾪삁愿(Greiner Bio-One, Monroe, NC, USA)쓽 젏쑀쑉씠 넂떎[4]. 吏꾧났梨꾪삁愿쓽 냼옱 泥④臾쇱 젣뭹쓽 쑀넻怨쇱젙씠 젣뭹쓽 뭹吏덉뿉 쁺뼢쓣 誘몄튌 닔 엳怨 寃곌낵쟻쑝濡 寃궗寃곌낵뿉 쁺뼢쓣 以 닔 엳쑝誘濡 援궡뿉꽌 깮궛릺怨 쑀넻맆 닔 엳뒗 吏꾧났梨꾪삁愿씠 젙솗븯怨 븞젙쟻씤 寃궗瑜 쐞븯뿬 븘슂븷 寃껋씠떎[5].

蹂 뿰援ъ뿉꽌뒗 씪諛섑솕븰寃궗 媛묒긽꽑샇瑜대が寃궗 珥 31媛吏 寃궗빆紐⑹뿉 븯뿬 援궛 吏꾧났梨꾪삁愿씤 V-Tube (AB Medical, Gwangju, Korea) 닔엯젣뭹씤 BD Vacutainer 諛 Greiner Vacuette瑜 鍮꾧탳遺꾩꽍븯뿬 꽦뒫쓣 룊媛븯떎.

옱猷 諛 諛⑸쾿

1. 뿰援щ긽

씠 뿰援щ뒗 2017뀈 11썡 븳 떖 룞븞 3媛쒖쓽 蹂묒썝뿉 궡썝븳 솚옄 삉뒗 뿰援ъ뿉 룞쓽븳 嫄닿컯븳 吏썝옄 벑 珥 100紐(뿬쓽룄꽦紐⑤퀝썝 20紐, 꽦鍮덉꽱듃蹂묒썝 40紐, 떊珥뚯꽭釉뚮뒪蹂묒썝 40紐)쓽 李몄뿬옄瑜 긽쑝濡 떆뻾븯떎. 긽옄뒗 紐⑤몢 18꽭 씠긽쓽 꽦씤뱾濡 紐⑤뱺 떆뻾怨쇱젙 媛 蹂묒썝쓽 엫긽뿰援ъ떖궗쐞썝쉶(institutional review board) 듅씤쓣 諛쏄퀬 룞쓽꽌瑜 諛쏆븘 吏꾪뻾븯떎(SC17DNDI0033).

2. 諛⑸쾿

1) 寃泥 梨꾩랬

吏썝옄瑜 긽쑝濡 젙留 梨꾩랬븳 삁븸쓣 씪諛섑솕븰寃궗 諛 媛묒긽꽑샇瑜대が寃궗瑜 쐞빐 serum separator tube (SST)뿉 떞븯떎. 吏꾧났梨꾪삁愿 needle holder (BD) multi draw 21G needle (Greiner)瑜 궗슜븯쑝硫, 梨꾪삁맂 삁븸쓣 媛곴컖 AB Medical궗쓽 SST V-Tube (V-Tube), BD Vacutainer SST Tube (BD-Tube), Greiner궗쓽 Vacuette SST Tube (Greiner-Tube)뿉 떞븯떎. 梨꾪삁 썑뿉뒗 梨꾪삁愿쓣 뿬윭 踰 遺뱶읇寃 쓷뱾뼱 쓳怨좏솢꽦젣(clot activator)뿉 옒 諛섏쓳븯룄濡 븯떎. 梨꾩랬맂 寃泥대뒗 梨꾪삁愿 젣議곗궗뿉꽌 沅뚭퀬븯뒗 궗슜踰뺤뿉 뵲씪 梨꾪삁 썑 15遺 샊 30遺 諛⑹튂 썑 3,000 g쑝濡 6遺꾧컙 썝떖遺꾨━븯쑝硫, 紐⑤뱺 삁븸 寃泥대뒗 梨꾪삁 썑 2 떆媛 씠궡뿉 寃궗瑜 吏꾪뻾븯떎. 삁泥 遺꾨━젮(separator gel) 쐞뿉 엳뒗 긽깭濡 寃궗븯떎. 梨꾪삁 떦씪 寃궗뒗 떎삩뿉 蹂닿븳 썑 梨꾪삁 썑 2떆媛 씠궡뿉 寃궗븯怨, 梨꾪삁 썑 1씪, 3씪, 7씪 寃궗뒗 4°C 깋옣蹂닿 썑 떆뻾븯떎.

2) 寃궗빆紐

寃궗빆紐⑹ 씪諛섑솕븰寃궗 以 븘뒪뙆뀒씠듃 븘誘몃끂쟾씠슚냼(aspartate aminotransferase, AST), 븣씪땶 븘誘몃끂쟾씠슚냼(alanine aminotransferase), 븣移쇰━꽦씤궛遺꾪빐슚냼(alkaline phosphatase), 媛먮쭏-湲猷⑦諛쟾씠슚냼(gamma-glutamyltransferase), 珥 떒諛(total protein), 븣遺誘(albumin), 삁븸슂냼吏덉냼(blood urea nitrogen), 겕젅븘떚땶(creatinine, Cr), 珥 鍮뚮━猷⑤퉰(total bilirubin), 吏곸젒鍮뚮━猷⑤퉰(direct bilirubin), 以묒꽦吏諛(triglyceride), 珥 肄쒕젅뒪뀒濡(total cholesterol), 怨좊룄吏떒諛 肄쒕젅뒪뀒濡(high-density lipoprotein cholesterol, HDL-C), 諛룄吏떒諛 肄쒕젅뒪뀒濡(low-density lipoprotein cholesterol, LDL-C), 룷룄떦(glucose), 슂궛(uric acid, UA), 移쇱뒛(calcium), 臾닿린씤(inorganic phosphorus), 겕젅븘떞궎굹젣(creatine kinase), 븘諛씪젣(amylase), 젚궛깉닔냼슚냼(lactate dehydrogenase, LD), 留덇렇꽕뒛(magnesium, Mg), 泥(iron), 遺덊룷솕泥좉껐빀뒫(unsaturated iron binding capacity), 由ы뙆젣(lipase), 굹듃瑜(sodium), 移쇰ⅷ(potassium, K), 뿼냼(chloride, Cl) 벑쓽 珥 28媛吏 빆紐⑷낵 媛묒긽꽑 샇瑜대が寃궗 珥쒿3 (triiodothyronine, T3), 쑀由촖4 (free thyroxine, free T4), 媛묒긽깦옄洹뱁샇瑜대が(thyroid-stimulating hormone, TSH) 벑 3媛吏 빆紐⑹쓣 떆뻾븯떎.

3) 李몄뿬湲곌蹂 遺꾩꽍옣鍮꾩 諛섎났 寃궗

李몄뿬湲곌蹂 李몄뿬 吏썝옄 닔뒗 뿬쓽룄꽦紐⑤퀝썝 20紐, 꽦鍮덉꽱듃蹂묒썝 40紐, 꽭釉뚮뒪蹂묒썝 40紐낆씠뿀怨, 洹 以 뿬쓽룄꽦紐⑤퀝썝쓽 20삁뒗 梨꾪삁 썑 2떆媛 씠궡(t=0 hours), 梨꾪삁 썑 24떆媛(t=24±2 hours), 梨꾪삁 썑 3씪(t=72±2 hours) 洹몃━怨 梨꾪삁 썑 7씪(t=168±2 hours)뿉 젣議곗궗蹂 媛곴컖쓽 tube濡 옱寃쓣 떎떆븯뿬 寃궗寃곌낵 李⑥씠瑜 鍮꾧탳븯떎. 뿬쓽룄꽦紐⑤퀝썝쓽 씪諛섑솕븰寃궗 옣鍮꾨뒗 Cobas c702 (Roche Diagnosis System, Basel, Switzerland), 媛묒긽꽑샇瑜대が寃궗 옣鍮꾨뒗 DXI 800 (Beckman Coulter, Brea, CA, USA)씠뿀怨 꽦鍮덉꽱듃뒗 AU5800 (Beckman Coulter), DXI 800 옣鍮 洹몃━怨 꽭釉뚮뒪蹂묒썝 Hitachi 7600 (Hitachi Hitechnologies, Tokyo, Japan)怨 Architect i2000sr analyzer (Abbot Laboratories, Abbot Park, IL, USA)떎.

3. 넻怨꾨텇꽍

V-Tube, BD-Tube, Greiner-Tube쓽 寃泥 梨꾩랬 썑 2떆媛 씠궡쓽 寃궗寃곌낵뒗 媛곴컖 빆紐⑸퀎濡 뙇泥대퉬援먮쾿쓣 궗슜븯뿬 鍮꾧탳遺꾩꽍븯떎. 媛 梨꾪삁愿蹂 寃곌낵媛믪쓽 긽愿愿怨꾨뒗 寃곌낵媛믪쓽 듅꽦뿉 뵲씪 simple correlation analysis 삉뒗 Spearman correlation analysis瑜 궗슜븯떎. 媛 梨꾪삁愿蹂 寃곌낵媛믪씠 몴以렪李⑥쓽 1.96諛 씠긽 굹뒗 李⑥씠瑜 Bland-Altman plot쓣 씠슜븯뿬 遺꾩꽍븯떎. 梨꾩랬 2떆媛 씠궡, 1씪, 3씪, 7씪쓽 鍮꾧탳瑜 쐞빐꽌 궇吏쒕퀎濡 뙇泥대퉬援먮쾿怨 諛섎났 痢≪젙 遺꾩궛遺꾩꽍(repeated measures analysis of variance)쓣 씠슜븯떎. 媛 뒠釉 媛 寃궗寃곌낵媛믪쓽 李⑥씠 鍮꾧탳뒗 Clinical Laboratory Improvement Amendments of 1988 (CLIA’88)媛 젣떆븯뒗 뿀슜踰붿쐞(allowable difference range)瑜 李멸퀬븯떎. 삉븳 Clinical and Laboratory Standards Institute GP34-A 吏移⑥뿉꽌 梨꾪삁愿 룊媛諛⑸쾿쑝濡 젣떆븯怨 엳뒗 엫긽쟻 뿀슜踰붿<뿉 븳 룊媛諛⑸쾿 以 븯굹씤 깮臾쇳븰쟻 蹂룞꽦(biological variability)쓣 寃궗寃곌낵瑜 鍮꾧탳븯뒗 뜲 李멸퀬븯怨[6], Westgard QC database뿉 洹쇨굅븳 뿀슜媛뒫李⑥씠(allowable difference, critical difference)뒗 Table 1뿉 젣떆븯떎[7]. 넻怨꾨텇꽍 넻怨꾪봽濡쒓렇옩씤 Analyse-it ver. 4.95 (Analyse-it Software Ltd., Leeds, UK)쓣 궗슜븯떎.

Table 1 . Statistical analysis of the analytes measured in samples collected in a V-Tube, a BD Vacuum Tube, and a Greiner Vacuum Tube.

   AnalytesV-TubeBD-TubeDifference %Pearson’s correlationGreiner- TubeDifference %Pearson’s correlationCritical difference (%)Allowable difference range
Albumin (g/dL)4.52±0.284.52±0.2800.9524.5±0.29–0.40.9451.07±10%
Alkaline phosphatase (IU/L)66.43±23.4466.56±23.410.20.99866.23±23.24–0.30.9902.15±30%
Alanine aminotransferase (U/L)20.48±17.5320.53±17.490.20.99020.63±17.400.70.9906.46±20%
Amylase (U/L)69.34±46.1469.53±46.170.30.99069.40±46.730.10.9902.90±30%
Aspartate aminotransferase (U/L)23.49±16.3423.46±16.28–0.10.99023.28±16.35–0.90.9904.10±20%
Blood urea nitrogen (mg/dL)12.9±2.812.99±2.860.70.99712.96±2.780.50.9994.03±9%
Calcium (mg/dL)9.46±0.349.44±0.36–0.20.9409.45±0.33–0.10.9500.70±1.0 mg/dL
Chloride (mmol/L)102.69±2.54102.81±2.590.10.980102.72±2.720.00.8760.40±5%
Creatine kinase (U/L)127.94±92.76128.24±91.650.20.999127.73±92.5–0.20.9997.60±30%
Creatinine (mg/dL)0.80±0.830.81±0.841.20.9990.81±0.831.20.9991.98±15%
Direct bilirubin (mg/dL)0.18±0.10.18±0.9900.9890.18±0.10.00.99012.26±30%
Free thyroxine (ng/dL)0.97±0.240.97±0.2400.9870.98±0.241.00.9801.90Target±3 SD
Glucose (mg/dL)106.89±32.90107.00±32.640.10.996106.95±32.470.10.9961.86±10%
High-density lipoprotein cholesterol (mg/dL)59.045±16.1358.05±16.70–1.70.99758.10±16.65–1.60.9962.43±30%
Iron(μg/dL)92.99±44.4893.59±44.560.60.99893.32±44.300.40.9998.83±20%
Lactate dehydrogenase (IU/L)187.19±39.54190.07±37.261.50.954185.79±36.68–0.80.6542.86±20%
Low-density lipoprotein cholesterol (mg/dL)109.37±33.41109.58±33.390.20.998122.23±46.0410.50.7122.60±30%
Lipase (U/L)33.75±16.9934.07±16.780.90.99833.82±16.940.20.99910.73±30%
Magnesium (mg/dL)1.52±0.581.49±0.57–2.00.9961.55±0.591.90.9971.20±25%
Phosphorus (mg/dL)3.68±0.383.70±0.400.50.9873.68±0.380.00.9862.71±12%
Potassium (mmol/L)4.28±0.334.21±0.32–1.70.8704.22±0.29–1.40.8401.53±0.5 mM
Sodium (mmol/L)140.61±1.67140.72±1.810.10.900140.66±2.030.00.6440.20±4 mM
Triiodothyronine (ng/mg)0.97±0.210.98±0.211.00.9800.98±0.211.00.9602.30Target±3 SD
Total bilirubin (mg/dL)0.66±0.310.66±0.320.00.9980.66±0.320.00.9977.26±20%
Cholesterol (mg/dL)187.32±35.43188.01±35.890.40.996187.87±35.970.30.9971.98±10%
Total protein (g/dL)7.24±0.397.25±0.4030.10.9737.24±0.390.00.9980.91±10%
Triglyceride (mg/dL)151.36±133.09151.71±1350.20.999152.06±136.120.50.9996.63±25%
Thyroid-stimulating hormone (mIU/L)1.98±2.171.94±2.03–2.10.9981.84±2.17–7.60.9906.43Target±3 SD
Unsaturated iron-binding capacity (μg/dL)233.65±68.22233.48±67.79–0.10.995233.29±67.59–0.20.996±25%
Uric acid (mg/dL)5.088±1.505.13±1.520.80.9985.096±1.510.20.9983.00±17%
Gamma-glutamyltransferase (U/L)29.83±30.8130.14±31.371.00.99829.89±30.770.20.9994.46±10%

Values are presented as mean±SD, unless otherwise stated. P-values were determined by using a paired t-test..

Abbreviation: SD, standard deviation..


寃 怨

100紐낆쓣 긽쑝濡 떆뻾븳 梨꾪삁 썑 2떆媛 씠궡뿉 痢≪젙븳 씪諛섑솕븰寃궗 媛묒긽꽑샇瑜대が寃궗뿉 븳 梨꾪삁愿 媛 鍮꾧탳뒗 Table 1뿉 젙由ы븯떎. 3媛쒖쓽 梨꾪삁愿 媛 寃곌낵媛믪쓽 李⑥씠뒗 紐⑤뱺 痢≪젙빆紐⑹뿉꽌 CLIA’88媛 젣떆븯뒗 뿀슜踰붿쐞瑜 留뚯”븯떎. 깮臾쇳븰쟻 蹂룞꽦뿉 洹쇨굅븳 뿀슜媛뒫李⑥씠(critical difference)瑜 踰쀬뼱궃 빆紐⑹ V-Tube BD-Tube뿉꽌뒗 Mg怨 K씠뿀怨, V-Tube Greiner-Tube뿉꽌뒗 LDL-C, Mg, TSH씠뿀떎. V-Tube BD-Tube쓽 寃곌낵媛믪쓣 뙇泥대퉬援먮쾿쓣 넻븯뿬 鍮꾧탳븯쓣 븣 쑀쓽븳 李⑥씠(P<0.05)瑜 蹂댁씤 빆紐⑹ Cr, HDL-C, iron, LD, lipase, Mg, K, total cholesterol 洹몃━怨 UA떎. 洹몃윭굹 씠뱾 빆紐⑹쓽 寃곌낵媛믪 媛뺥븳 뼇쓽 꽑삎 긽愿愿怨꾨 蹂댁떎. 씠뱾 빆紐 以 긽愿怨꾩닔媛 媛옣 궙 빆紐⑹ potassium씠뿀떎(rho竊0.870). V-Tube Greiner-Tube 寃곌낵媛믪쓣 뙇泥대퉬援먮쾿쓣 넻븯뿬 鍮꾧탳븯쓣 븣 쑀쓽븳 李⑥씠瑜 蹂댁씤 빆紐⑹ AST, direct-bilirubin, HDL-C, LDL-C, Mg, K 洹몃━怨 TSH쑝굹 紐⑤몢 媛뺥븳 뼇쓽 꽑삎 긽愿愿怨꾨 蹂댁怨 媛옣 궙 긽愿怨꾩닔瑜 蹂댁씤 빆紐⑹ LDL-C떎(rho竊0.712). BD-Tube Greiner-Tube 媛 쑀쓽븳 李⑥씠瑜 蹂댁씤 빆紐⑹ Cr, direct bilirubin, T4, LD, LDL-C, lipase, Mg, T3, total bilirubin, TSH, UA 벑 11媛 빆紐⑹씠뿀떎.

媛 梨꾪삁愿쓽 寃곌낵媛 李⑥씠瑜 Bland-Altman plot쑝濡 遺꾩꽍븳 寃곌낵, 젣議곗궗蹂 寃곌낵媛믪씠 몴以렪李 1.96諛 씠긽쑝濡 利앷븳 寃쎌슦뒗 媛 痢≪젙빆紐⑹뿉 뵲씪 쟻寃뚮뒗 1媛쒖뿉꽌 留롪쾶뒗 10媛쒖쓽 寃泥닿퉴吏 엳뿀떎. Bland-Altman plot瑜 踰쀬뼱굹뒗 寃곌낵뒗 媛곴컖쓽 寃궗빆紐⑸떦 룊洹 V-Tube/BD-Tube 4.9嫄, V-Tube/Greiner-Tube 4.6嫄, BD-Tube/Greiner-Tube 5.1嫄댁씠뿀怨 젣議곗궗 媛 쑀쓽븳 李⑥씠뒗 뾾뿀떎. 떎瑜 빆紐⑹뿉 鍮꾪븯뿬 鍮꾧탳쟻 궙 긽愿怨꾩닔瑜 蹂댁씤 쟾빐吏 寃궗빆紐⑹쓽 Bland-Altman plot 洹몃옒봽뒗 Fig. 1뿉 굹궡뿀떎. 쟾빐吏덇궗쓽 媛 빆紐⑸퀎 뒠釉 젣議곗궗蹂 痢≪젙寃곌낵媛믪쓽 듅씠젏 뾾뿀떎. 鍮꾧탳쟻 궙 긽愿怨꾩닔瑜 蹂댁씤 LD LDL-C 빆紐⑹쓽 Bland-Altman plot 洹몃옒봽뒗 Fig. 2뿉 굹뿴븯떎. 빟 125 mg/dL 씠긽쓽 LDL-C瑜 痢≪젙븯뒗 뜲 엳뼱꽌 Greiner-Tube瑜 궗슜븳 寃곌낵媛믨낵 V-Tube굹 BD-Tube瑜 궗슜븳 寃곌낵媛 궗씠뿉 李⑥씠젏쓣 蹂댁떎. 媛묒긽꽑湲곕뒫寃궗쓽 Bland-Altman plot 洹몃옒봽瑜 Fig. 3뿉 굹궡뿀떎. TSH 痢≪젙뿉 엳뼱꽌 Architect i2000sr analyzer 옣鍮꾩 Greiner-Tube瑜 궗슜븳 寃궗쓽 寃곌낵媛믪씠 떎瑜 tube瑜 궗슜븳 寃곌낵媛믨낵 鍮꾧탳븯뿬 李⑥씠젏쓣 蹂댁떎.

Figure 1.

Bland-Altman plot of the chemical tests in samples collected in V-Tubes, BD Vacutainer and Greiner Vacuette (A, sodium; B, potassium; C, chloride). Abbreviation: SD, standard deviation.


Figure 2.

Bland-Altman plot of the chemical tests in samples collected in V-Tubes, BD Vacutainer and Greiner Vacuette (A, LD; B, LDL-C). Abbreviations: SD, standard deviation; LD, lactate dehydrogenase; LDL-C, low-density lipoprotein cholesterol.


Figure 3.

Bland-Altman plot of the chemical tests in samples collected in V-Tubes, BD Vacutainer, and Greiner Vacuette (A, free T4; B, T3; C, TSH). Abbreviations: SD, standard deviation; T3, triiodothyronine; free T4, free thyroxine; TSH, thyroid-stimulating hormone.


媛 젣議곗궗蹂 tube쓽 梨꾪삁 떦씪(day 0), 梨꾪삁 썑 븯猷(day 1), 梨꾪삁 썑 3씪(day 3), 梨꾪삁 썑 7씪(day 7)쓽 寃곌낵媛믪쓣 鍮꾧탳븯떎(Tables 2-4). 梨꾪삁 떦씪怨 媛 궇吏쒕퀎 옱寃寃곌낵媛믪쓽 李⑥씠媛 넻怨꾩쟻쑝濡 쑀쓽븯吏 븡 빆紐⑹ V-Tube뿉꽌뒗 Cl, BD-Tube뿉꽌뒗 AST 빆紐⑸퓧씠뿀怨, Greiner-Tube뿉꽌뒗 뾾뿀떎. 梨꾪삁 떦씪쓽 寃궗寃곌낵媛믨낵 媛옣 겙 李⑥씠瑜 蹂댁씠뒗 궇吏쒕뒗 梨꾪삁 썑 븯猷⑥쓽 寃궗寃곌낵媛믪씠뿀떎. 梨꾪삁 떦씪(day 0)怨 븯猷(day 1) 寃곌낵媛믪씠 넻怨꾩쟻쑝濡 쑀쓽븳 李⑥씠瑜 蹂댁씠뒗 빆紐⑹ V-Tube뿉꽌뒗 26빆紐, BD-Tube뿉꽌뒗 23빆紐, Greiner-Tube뿉꽌뒗 26빆紐⑹씠뿀떎. 梨꾪삁 떦씪 寃곌낵媛믪쓣 湲곗쑝濡 븯쓣 븣 媛 tube 젣議곗궗蹂 씪諛섑솕븰寃궗 諛 媛묒긽꽑샇瑜대が寃궗쓽 쟾泥 룊洹 寃곌낵媛믪쓽 理쒕 蹂룞룺 V-Tube媛 1.1%, BD-Tube 1.48%, Greiner-Tube 1.46%濡 V-Tube媛 긽쟻쑝濡 븞젙쟻씤 蹂룞룺쓣 蹂댁떎. 빆紐⑸퀎濡쒕뒗 V-Tube뿉꽌뒗 AST媛, BD-Tube Greiner-Tube뿉꽌뒗 LD, Mg, T3 빆紐⑹씠 떎瑜 tube뿉 鍮꾪븯뿬 겙 蹂룞룺쓣 蹂댁떎.

Table 2 . Daily variation in clinical chemistry assay results between days 0, 1, 3, and 7 obtained from blood collected in V-Tubes.

   AnalytesDay 0 vs. day 1Day 0 vs. day 3Day 0 vs. day 7Day 1 vs. day 7




Mean bias (95% CI)P- valueMean bias (95% CI)P- valueMean bias (95% CI)P- valueMean bias (95% CI)P- value
Albumin (g/dL)–0.09 (–0.13 to 0.04)0.001–0.04 (–0.11 to 0.03)0.2470.002 (–0.04 to 0.05)0.9240.09 (0.03 to 0.15)0.004
Alkaline phosphatase (IU/L)–2.55 (–3.23 to –1.86)<0.0010.05 (–0.81 to 0.91)0.905–0.55 (–1.31 to 0.22)0.1502.0 (1.37 to 2.62)<0.001
Alanine aminotransferase (U/L)0.6 (0.08 to 1.11)0.024–0.7 (–1.18 to –0.21)0.0070.2 (–0.73 to 1.13)0.658–0.4 (–1.07 to 0.27)0.226
Amylase (U/L)–1.3 (–1.60 to –0.99)<0.0010.6 (–0.06 to 1.26)0.0760.4 (0.04 to 0.75)0.0281.7 (1.29 to 2.1)<0.001
Aspartate aminotransferase (U/L)1.85 (1.13 to 2.56)<0.001–0.15 (–1.01 to 0.71)0.7200.25 (–0.62 to 1.12)0.555–1.60 (–2.30 to –0.89)<0.001
Blood urea nitrogen (mg/dL)–0.46 (–0.59 to –0.32)<0.001–0.33 (–0.47 to –0.19)<0.0010.03 (–0.06 to 0.13)0.4720.49 (0.32 to 0.66)<0.001
Calcium (mg/dL)–0.24 (–0.32 to –0.17)<0.0010.48 (–0.54 to 1.49)0.336–0.06 (–0.12 to –0.01)0.0190.18 (0.1 to 0.25)<0.001
Chloride (mmol/L)–0.3 (–0.6 to 0.01)0.055–0.25 (–0.7 to 0.2)0.2620.2 (–0.19 to 0.59)0.2970.5 (0.14 to 0.85)0.008
Creatine kinase (U/L)–2.15 (–2.78 to –1.51)<0.0010.25 (–1.28 to 1.78)0.737–0.15 (–1.40 to 1.10)0.7372.0 (0.53 to 3.46)0.01
Creatinine (mg/dL)–0.05 (–0.07 to –0.03)<0.001–0.03 (–0.04 to –0.02)<0.001–0.04 (–0.05 to –0.02)<0.0010.01 (–0.01 to 0.03)0.221
Direct bilirubin (mg/dL)0.007 (0.001 to 0.01)0.0280.01 (0.001 to 0.019)0.0420.024 (0.012 to 0.035)<0.0010.01 (0.001 to 0.02)0.004
Free thyroxine (ng/dL)0.009 (–0.01 to 0.02)0.3430.04 (0.02 to 0.05)<0.0010.009 (–0.003 to 0.021)0.149–0.001 (–0.02 to 0.01)0.960
Glucose (mg/dL)–0.3 (–1.09 to 0.49)0.437–1.05 (–2.06 to –0.03)0.043–1.1 (–2.16 to –0.03)0.043–0.8 (–1.69 to 0.09)0.076
High-density lipoprotein cholesterol (mg/dL)–1.01 (–1.32 to –0.69)<0.0010.97 (0.28 to 1.66)0.008–0.43 (–1.45 to 0.59)0.3910.58 (–0.45 to 1.61)0.255
Iron(μg/dL)–2.1 (–3.04 to –1.15)<0.001–2.25 (–3.03 to –1.46)<0.001–2.4 (–3.4 to –1.35)<0.001–0.3 (–1.21 to 0.61)0.500
Lactate dehydrogenase (IU/L)–1.85 (–2.81 to –0.88)0.0012.7 (–3.5 to 8.92)0.3756.1 (0.46 to 11.73)0.0350.795 (2.53 to 13.36)0.006
Low-density lipoprotein cholesterol (mg/dL)0.2 (–0.52 to 0.92)0.5691.90 (0.33 to 3.46)0.0204.15 (3.22 to 5.07)<0.0013.95 (2.92 to 4.97)<0.001
Lipase (U/L)0.12 (–0.07 to 0.32)0.2070.59 (0.3 to 0.88)<0.001–0.99 (–1.49 to –0.49)0.001–1.12 (–1.65 to –0.58)<0.001
Magnesium (mg/dL)–0.12 (–0.14 to –0.09)<0.001–0.06 (–0.08 to –0.03)<0.001–0.21 (–0.23 to –0.18)<0.001–0.09 (–0.11 to –0.06)<0.001
Phosphorus (mg/dL)–0.18 (–0.21 to –0.15)<0.001–0.06 (–0.11 to –0.01)0.019–0.13 (–0.16 to –0.09)<0.0010.05 (0.02 to 0.08)0.002
Potassium (mmol/L)–0.05 (–0.07 to –0.02)<0.001–0.05 (–0.11 to 0.001)0.053–0.26 (–0.32 to –0.2)<0.001–0.21 (–0.27 to –0.15)<0.001
Sodium (mmol/L)–1.4 (–1.63 to –1.16)<0.0010.0 (–0.33 to 0.33)1.000–1.85 (–2.28 to –1.41)<0.001–0.45 (–0.8 to –0.09)0.016
Triiodothyronine (ng/mg)0.06 (0.04 to 0.07)<0.0010.03 (0.01 to 0.05)0.0010.13 (0.11 to 0.14)<0.0010.07 (0.06 to 0.08)<0.001
Total bilirubin (mg/dL)0.03 (0.02 to 0.04)<0.001–0.002 (–0.01 to 0.007)0.5920.03 (0.024 to 0.05)<0.0010.006 (–0.005 to 0.017)0.285
Cholesterol (mg/dL)–5.0 (–6.8 to –3.15)<0.001–3.5 (–5.74 to –1.25)0.004–4.65 (–6.1 to –3.1)<0.0010.35 (–1.2 to 1.9)0.644
Total protein (g/dL)–0.06 (–0.11 to –0.01)0.0180.1 (0.05 to 0.15)<0.0010.11 (0.08 to 0.14)<0.0010.17 (0.12 to 0.23)<0.001
Triglyceride (mg/dL)–6.4 (–7.89 to –4.9)<0.001–3.3 (–5.1 to –1.44)0.001–2.95 (–3.58 to –2.31)<0.0013.45 (2.13 to 4.76)<0.001
Thyroid-stimulating hormone (mIU/L)–0.87 (–0.12 to –0.04)<0.001–0.032 (–0.06 to 0.001)0.055–0.023 (–0.04 to –0.003)0.0240.064 (0.036 to 0.09)<0.001
Unsaturated iron-binding capacity (μg/dL)4.05 (1.1 to 6.99)0.0105.15 (0.93 to 9.36)0.019–10.55 (–13.84 to –7.25)<0.001–14.6 (–18.04 to –11.15)<0.001
Uric acid (mg/dL)–0.17 (–0.21 to –0.13)<0.001–0.05 (–0.09 to –0.003)0.038–0.08 (–0.12 to –0.04)<0.0010.09 (0.06 to 0.12)<0.001
Gamma-glutamyltransferase (U/L)–0.75 (–1.22 to –0.27)0.004–0.20 (–0.69 to 0.29)0.408–0.45 (–1.01 to 0.11)0.1070.3 (–0.10 to 0.70)0.137

P-values were determined by using a paired t-test..

Abbreviation: CI, confidence interval..


Table 3 . Daily variation in clinical chemistry assay results between days 0, 1, 3, and 7 obtained from blood collected in BD-Tubes.

   AnalytesDay 0 vs. day 1Day 0 vs. day 3Day 0 vs. day 7Day 1 vs. day 7




Mean bias (95% CI)P- valueMean bias (95% CI)P- valueMean bias (95% CI)P- valueMean bias (95% CI)P- value
Albumin (g/dL)–0.12 (–0.18 to –0.06)<0.001–0.02 (–0.08 to 0.03)0.3920.01 (–0.04 to 0.07)0.5920.14 (0.08 to 0.19)<0.001
Alkaline phosphatase (IU/L)–2.75 (–3.2 to –2.07)<0.001–0.15 (–1.24 to 0.94)0.776–0.9 (–1.56 to –0.24)0.0101.85 (1.12 to 2.58)<0.001
Alanine aminotransferase (U/L)0.3 (–0.27 to 0.87)0.285–0.55 (–1.02 to –0.08)0.0240.3 (–0.23 to 0.8)0.2490 (–0.4 to 0.4)1.000
Amylase (U/L)–0.95 (–1.41 to –0.48)<0.0010.6 (–0.15 to 1.35)0.1100.3 (–0.38 to 0.98)0.3681.25 (0.74 to 1.75)<0.001
Aspartate aminotransferase (U/L)0.65 (–0.21 to 1.51)0.131–0.15 (–0.63 to 0.34)0.5270.1 (–0.5 to 0.70)0.733–0.55 (–1.5 to 0.4)0.242
Blood urea nitrogen (mg/dL)–0.42 (–0.58 to –0.27)<0.001–0.21 (–0.33 to –0.09)0.001–0.01 (–0.15 to 0.14)0.9470.42 (0.22 to 0.61)<0.001
Calcium (mg/dL)–0.28 (–0.36 to –0.20)<0.0010.01 (–0.06 to 0.07)0.878–0.07 (–0.13 to –0.01)0.0270.21 (0.15 to 0.27)<0.001
Chloride (mmol/L)–0.15 (–0.49 to 0.19)0.3790.15 (–0.31 to 0.61)0.5050.7 (0.24 to 1.15)0.0050.85 (0.34 to 1.34)0.002
Creatine kinase (U/L)–1.95 (–2.7 to –1.1)<0.0010.7 (–0.61 to 2.01)0.2790.15 (–1.23 to 1.53)0.8232.1 (0.68 to 3.51)0.006
Creatinine (mg/dL)–0.06 (–0.08 to –0.04)<0.001–0.04 (–0.05 to –0.03)<0.001–0.04 (–0.06 to –0.03)<0.0010.01 (–0.01 to 0.04)0.188
Direct bilirubin (mg/dL)0.007 (0.002 to 0.01)0.010–0.001 (–0.001 to 0.006)0.8830.01 (0.004 to 0.015)0.0010.002 (–0.004 to 0.009)0.460
Free thyroxine (ng/dL)–0.003 (–0.02 to 0.01)0.7600.03 (0.015 to 0.04)0.0010.006 (–0.01 to 0.02)0.4840.009 (–0.01 to 0.03)0.413
Glucose (mg/dL)–1.05 (–2.03 to –0.06)0.039–0.45 (–1.18 to 0.28)0.216–1.0 (–2.29 to 0.29)0.1230.05 (–0.82 to 0.92)0.906
High-density lipoprotein cholesterol (mg/dL)–0.29 (–0.82 to 0.24)0.2731.38 (0.88 to 1.88)<0.0010.48 (–0.44 to 1.40)0.2900.77 (0.04 to 1.49)0.038
Iron(μg/dL)–2.35 (–3.22 to –1.47)<0.001–1.95 (–2.77 to –1.12)<0.001–2.1 (–2.7 to –1.4)<0.0010.25 (–0.56 to 1.06)0.529
Lactate dehydrogenase (IU/L)–2.6 (–4.21 to –0.98)0.00310.8 (6.96 to 14.63)<0.00111.8 (7.13 to 16.46)<0.00114.4 (9.24 to 19.55)<0.001
Low-density lipoprotein cholesterol (mg/dL)–0.65 (–1.56 to 0.26)0.1531.25 (–0.5 to 3.0)0.1532.95 (2.12 to 3.77)<0.0013.60 (2.76 to 4.43)<0.001
Lipase (U/L)–0.2 (–0.5 to 0.1)0.1890.5 (0.16 to 0.83)0.005–1.18 (–1.64 to 0.71)<0.001–0.98 (–1.29 to –0.66)<0.001
Magnesium (mg/dL)–0.11 (–0.14 to –0.08)<0.001–0.03 (–0.05 to –0.003)0.030–0.17 (–0.19 to –0.14)<0.001–0.05 (–0.07 to –0.03)<0.001
Phosphorus (mg/dL)–0.19 (–0.22 to –0.16)<0.001–0.04 (–0.07 to –0.008)0.017–0.065 (–0.1 to –0.02)0.0020.13 (0.09 to 0.16)<0.001
Potassium (mmol/L)–0.05 (–0.07 to –0.03)<0.0010.07 (0.03 to 0.11)0.001–0.005 (–0.04 to 0.039)0.8150.05 (0.005 to 0.094)0.029
Sodium (mmol/L)–1.4 (–1.63 to –1.16)<0.0010.5 (0.25 to 0.74)<0.001–1.75 (–2.04 to –1.45)<0.001–0.35 (–0.62 to –0.07)0.015
Triiodothyronine (ng/mg)0.05 (0.03 to 0.07)<0.0010.03 (0.01 to 0.05)0.0030.12 (0.11 to 0.14)<0.0010.069 (0.05 to 0.08)<0.001
Total bilirubin (mg/dL)0.02 (0.013 to 0.039)<0.001–0.006 (–0.02 to 0.007)0.3500.03 (0.01 to 0.04)<0.0010.007 (–0.003 to 0.017)0.167
Cholesterol (mg/dL)–4.55 (–6.9 to –2.1)0.001–3.2 (–5.35 to –1.04)0.006–3.95 (–6.29 to –1.6)0.0020.6 (–1.29 to 2.49)0.515
Total protein (g/dL)–0.09 (–0.14 to –0.03)0.0020.1 (0.03 to 0.16)0.0040.09 (0.03 to 0.15)0.0020.18 (0.13 to 0.24)<0.001
Triglyceride (mg/dL)–6.25 (–7.87 to –4.62)<0.001–2.75 (–4.02 to –1.47)<0.001–1.8 (–3.21 to –0.38)0.0154.45 (2.74 to 6.15)<0.001
Thyroid-stimulating hormone (mIU/L)–0.1 (–0.13 to –0.07)<0.001–0.03 (–0.05 to –0.007)0.014–0.04 (–0.06 to –0.01)0.0070.063 (0.04 to 0.08)<0.001
Unsaturated iron-binding capacity (μg/dL)0.6 (–3.34 to 4.54)0.7536.2 (3.19 to 9.20)<0.001–11.1 (–15.04 to –7.15)<0.001–11.7 (–15.83 to –7.56)<0.001
Uric acid (mg/dL)–0.17 (–0.22 to –0.11)<0.001–0.03 (–0.07 to 0.005)0.090–0.06 (–0.10 to –0.011)0.0190.11 (0.064 to 0.15)<0.001
Gamma-glutamyltransferase (U/L)–0.55 (–1.06 to –0.03)0.037–0.05 (–0.56 to 0.46)0.841–0.45 (–0.91 to 0.01)0.0580.1 (–0.35 to 0.55)0.649

P-values were determined by using a paired t-test..

Abbreviation: CI, confidence interval..


Table 4 . Daily variation in clinical chemistry assay results between days 0, 1, 3, and 7 obtained from blood collected in Greiner-Tubes.

   AnalytesDay 0 vs. day 1Day 0 vs. day 3Day 0 vs. day 7Day 1 vs. day 7




Mean bias (95% CI)P- valueMean bias (95% CI)P- valueMean bias (95% CI)P- valueMean bias (95% CI)P- value
Albumin (g/dL)–0.11 (–0.16 to –0.05)0.001–0.05 (–0.11 to 0.01)0.0720.01 (–0.05 to 0.07)0.7480.12 (0.05 to 0.18)0.001
Alkaline phosphatase (IU/L)–2.8 (–3.49 to –2.11)<0.001–0.2 (–1.24 to 0.84)0.694–0.55 (–1.44 to 0.34)0.2122.25 (1.39 to 3.10)<0.001
Alanine aminotransferase (U/L)0.7 (0.27 to1.13)0.003–0.1 (–0.49 to 0.29)0.6060.75 (0.32 to 1.17)0.0020.05 (–0.39 to 0.49)0.815
Amylase (U/L)–1.5 (–1.82 to –1.17)<0.0010.7 (0.1 to 1.29)0.0230.75 (0.32 to 1.17)0.0022.25 (1.68 to 2.81)<0.001
Aspartate aminotransferase (U/L)0.9 (0.2 to 1.59)0.0140.2 (–0.58 to 0.98)0.599–0.2 (–1.02 to 0.62)0.618–1.1 (–1.97 to –0.23)0.016
Blood urea nitrogen (mg/dL)–0.43 (–0.58 to –0.28)<0.001–0.19 (–0.31 to –0.07)0.004–0.05 (–0.16 to 0.06)0.3530.38 (0.2 to 0.55)<0.001
Calcium (mg/dL)–0.23 (–0.30 to –0.16)<0.0010.01 (–0.04 to 0.06)0.6940.01 (–0.05 to 0.07)0.7660.24 (0.17 to 0.31)<0.001
Chloride (mmol/L)–0.45 (–0.77 to –0.13)0.009–0.05 (–0.49 to 0.39)0.8150.05 (–0.5 to 0.6)0.8530.5 (–0.03 to 1.03)0.066
Creatine kinase (U/L)–2.15 (–2.86 to –1.43)<0.0011.1 (–0.34 to 2.54)0.1260.35 (–0.41 to 1.11)0.3492.5 (1.96 to 3.03)<0.001
Creatinine (mg/dL)–0.05 (–0.07 to –0.030)<0.001–0.03 (–0.05 to –0.02)<0.001–0.03 (–0.48 to –0.02)<0.0010.01 (–0.01 to 0.03)0.092
Direct bilirubin (mg/dL)0.004 (–0.001 to 0.009)0.1190.004 (–0.005 to 0.014)0.3590.015 (0.004 to 0.026)0.0090.01 (–0.0001 to 0.02)0.053
Free thyroxine (ng/dL)0.001 (–0.017 to 0.02)0.8670.03 (0.02 to 0.054)<0.0010.013 (–0.004 to 0.03)0.1420.01 (–0.008 to 0.031)0.248
Glucose (mg/dL)–1.1 (–2.1 to –0.06)0.039–1.8 (–2.68 to –0.91)<0.001–2.05 (–3.2 to –0.89)0.001–0.95 (–1.89 to –0.008)0.048
High-density lipoprotein cholesterol (mg/dL)–0.71 (–1.32 to –0.1)0.0251.46 (1.07 to 1.84)<0.0010.46 (–0.23 to 1.15)0.1851.17 (0.34 to 2.00)0.008
Iron(μg/dL)–2.1 (–3.13 to –1.06)<0.001–1.05 (–1.60 to –0.49)0.001–1.6 (–2.62 to –0.57)0.0040.5 (–0.75 to 1.75)0.415
Lactate dehydrogenase (IU/L)–2.3 (–3.96 to –0.6)0.0099.0 (2.62 to 15.38)0.00813.7 (7.00 to 20.39)<0.00116.0 (9.27 to 22.72)<0.001
Low-density lipoprotein cholesterol (mg/dL)–0.65 (–1.56 to 0.26)0.1532.35 (0.36 to 4.33)0.0233.8 (2.85 to 4.74)<0.0014.45 (3.68 to 5.21)<0.001
Lipase (U/L)0.07 (–0.11 to 0.26)0.4220.44 (0.09 to 0.79)0.014–1.11 (–1.67 to 0.55)0.001–1.19 (–1.72 to –0.65)<0.001
Magnesium (mg/dL)–0.1 (–0.12 to –0.08)<0.001–0.03 (–0.05 to –0.003)0.030–0.17 (–0.19 to –0.14)<0.001–0.06 (–0.09 to –0.03)<0.001
Phosphorus (mg/dL)–0.2 (–0.23 to –0.16)<0.001–0.06 (–0.11 to –0.01)0.012–0.12 (–0.17 to –0.07)<0.0010.07 (0.04 to 0.10)<0.001
Potassium (mmol/L)–0.06 (–0.08 to –0.03)<0.001–0.005 (–0.06 to 0.05)0.858–0.33 (–0.40 to –0.25)<0.001–0.27 (–0.33 to –0.2)<0.001
Sodium (mmol/L)–1.65 (–1.87 to –1.42)<0.0010.15 (–0.25 to 0.55)0.453–1.5 (–2.4 to –0.59)0.0030.15 (–0.75 to 1.05)0.732
Triiodothyronine (ng/mg)0.06 (0.049 to 0.076)<0.0010.04 (0.02 to 0.06)<0.0010.13 (0.12 to 0.15)<0.0010.07 (0.05 to 0.09)<0.001
Total bilirubin (mg/dL)0.02 (0.004 to 0.04)0.018–0.007 (–0.02 to 0.005)0.2410.043 (0.025 to 0.06)<0.0010.02 (0.008 to 0.032)0.002
Cholesterol (mg/dL)–3.75 (–5.31 to –2.1)<0.001–3.2 (–5.0 to –1.39)0.001–3.4 (–4.89 to –1.90)<0.0010.35 (–1.64 to 2.34)0.717
Total protein (g/dL)–0.05 (–0.1 to –0.01)0.0100.14 (0.09 to 0.19)<0.0010.12 (0.09 to 0.16)<0.0010.18 (0.13 to 0.23)<0.001
Triglyceride (mg/dL)–6.7 (–8.13 to –5.26)<0.001–2.65 (–4.27 to –1.02)0.003–2.9 (–3.88 to –1.91)<0.0013.8 (2.3 to 5.29)<0.001
Thyroid-stimulating hormone (mIU/L)–0.1 (–0.13 to –0.08)<0.001–0.027 (–0.05 to 0.001)0.047–0.03 (–0.06 to –0.01)0.0080.07 (0.04 to 0.10)<0.001
Unsaturated iron-binding capacity (μg/dL)0.75 (–2.07 to 3.57)0.5856.60 (2.48 to 10.71)0.003–10.4 (–14.62 to –6.17)<0.001–11.15 (–15.04 to –7.25)<0.001
Uric acid (mg/dL)–0.16 (–0.20 to –0.12)<0.001–0.065 (–0.10 to –0.02)0.004–0.07 (–0.1 to –0.03)<0.0010.09 (0.05 to 0.13)<0.001
Gamma-glutamyltransferase (U/L)–0.75 (–1.14 to –0.35)0.001–0.35 (–0.98 to 0.28)0.260–0.5 (–1.05 to 0.05)0.0760.25 (–0.17 to 0.67)0.234

P-values were determined by using a paired t-test..

Abbreviation: CI, confidence interval..


媛 李몄뿬湲곌蹂 V-Tube BD-Tube, Greiner-Tube쓽 寃곌낵媛믪쓽 李⑥씠뒗 Table 5뿉 젙由ы븯쑝硫 3怨녹쓽 李몄뿬湲곌뿉꽌 떆뻾븳 遺遺 빆紐⑹뿉꽌 媛 tube蹂 寃곌낵媛믪쓽 李⑥씠뒗 뿀슜踰붿쐞 씠궡濡 痢≪젙릺뿀떎.

Table 5 . Rate of difference (%) of measured analytes between blood collected in V-Tube and BD-Tube, and V-Tube and Greiner-Tube.

  AnalytesYeouido St. Mary’s HospitalSt. Vincent’s HospitalSeverance HospitalCritical difference (%)Allowable difference range




BD-TubeGreiner-TubeBD-TubeGreiner-TubeBD-TubeGreiner-Tube
Albumin0.050.330.00.50.0110.921.07±10%
Alkaline phosphatase0.040.040.150.290.220.02.15±30%
Alanine aminotransferase0.171.570.080.480.330.226.46±20%
Amylase0.330.430.20.040.030.262.90±30%
Aspartate aminotransferase0.550.410.250.090.991.884.10±20%
Blood urea nitrogen0.10.020.190.840.140.234.03±9%
Calcium0.030.160.040.030.210.640.70±1.0 mg/dL
Chloride0.050.070.290.120.050.030.40±5%
Creatine kinase0.510.510.080.380.101.297.60±30%
Creatinine1.751.070.020.391.191.041.98±15%
Direct bilirubin1.61.260.016.932.050.4812.26±30%
Free thyroxine0.040.310.010.760.691.861.90Target±3 SD
Glucose0.030.11.080.670.720.631.86±10%
High-density lipoprotein cholesterol1.851.190.51.051.361.712.43±30%
Iron1.00.970.080.210.60.228.83±20%
Lactate dehydrogenase1.981.066.00.221.192.832.86±20%
Low-density lipoprotein cholesterol0.550.880.330.20.180.232.60±30%
Lipase0.120.130.831.410.00.1910.73±30%
Magnesium1.692.250.040.70.561.571.20±25%
Phosphorus0.20.00.00.070.140.632.71±12%
Potassium0.631.210.050.211.961.841.53±0.5 mM
Sodium0.020.090.040.10.220.070.20±4 mM
Triiodothyronine0.690.290.01.421.450.862.30Target±3 SD
Total bilirubin0.050.820.010.390.490.697.26±20%
Cholesterol0.110.721.080.260.240.271.98±10%
Total protein0.040.440.020.280.070.780.91±10%
Triglyceride0.230.440.150.10.360.536.63±25%
Thyroid-stimulating hormone0.640.210.080.870.2520.176.43Target±3 SD
Unsaturated iron-binding capacity0.080.32.00.190.990.6±25%
Uric acid0.30.30.060.080.910.193.00±17%
Gamma glutamyl transpeptidase00.160.030.562.150.074.46±10%

Abbreviation: SD, standard deviation..


怨 李

吏꾧났梨꾪삁愿 援ъ꽦옱猷뚯 泥④臾쇱뿉 쓽빐꽌 寃궗 쟾 삤瑜섏뿉 쁺뼢쓣 誘몄튌 닔 엳뒗 젙諛꽦쓣 슂援ы븯뒗 쓽猷뚭린湲곗쓽 씪醫낆씠떎[1-3]. 삉븳 옄泥댁쓽 吏꾧났뒫젰怨 뭹吏덇由 삉븳 寃궗寃곌낵뿉 쁺뼢쓣 以 닔 엳쑝硫 씠뿉 븳 留롮 뿰援ш 엳뿀떎[5,8]. 쁽옱 援궡뿉꽌 궗슜릺怨 엳뒗 吏꾧났梨꾪삁愿 BD Greiner 닔엯젣뭹씠 遺遺꾩쓣 李⑥븯怨 엳怨 援궛젣뭹쓽 떆옣젏쑀쑉 궙 렪씠떎. 닔엯 쑀넻怨쇱젙뿉꽌 諛쒖깮븷 닔 엳뒗 뭹吏덈Ц젣 洹몄뿉 뵲瑜 寃궗寃곌낵쓽 삤瑜 벑쓽 媛뒫꽦쓣 怨좊젮븷 븣 援궛젣뭹쓣 寃궗떎뿉꽌 궗슜븳떎뒗 寃껋 異⑸텇엳 洹 슚슜꽦씠 엳쓣 寃껋씠떎. 洹몃윭굹 寃궗寃곌낵뿉 겙 쁺뼢쓣 誘몄튌 닔 엳뒗 젙諛븳 쓽猷뚭린湲곕씪뒗 젏뿉꽌 吏꾧났梨꾪삁愿쓽 寃궗쓽 젙솗꽦 諛 븞젙꽦뿉 븳 寃利앹 븘닔쟻씠떎. 蹂 뿰援ъ뿉꽌뒗 援궡 媛쒕컻맂 吏꾧났梨꾪삁愿 V-Tube 꽭怨 떆옣뿉꽌 꼸由 궗슜릺怨 엳뒗 BD Greiner 쉶궗젣뭹쓽 꽦뒫鍮꾧탳瑜 3媛 쓽猷뚭린愿씠 李몄뿬븯뿬 떎뼇븳 寃궗옣鍮 諛 寃궗솚寃쎌뿉 끂異쒗븯뿬 룊媛븷 닔 엳뿀떎.

3媛 젣議곗궗쓽 梨꾪삁愿 媛 씪諛섑솕븰寃궗 諛 媛묒긽꽑샇瑜대が寃궗쓽 寃곌낵媛믪쓽 李⑥씠뒗 CLIA’88쓽 뿀슜李 踰붿쐞瑜 紐⑤몢 留뚯”븯쑝硫, 깮臾쇳븰쟻 蹂룞꽦뿉 洹쇨굅븳 뿀슜媛뒫李⑥씠(critical difference)瑜 遺遺 留뚯”븯떎. 媛 tube蹂 뙇泥대퉬援먮쾿쓣 넻븳 寃곌낵 鍮꾧탳뿉꽌뒗 V-Tube BD-Tube쓽 Cr, HDL-C, iron, LD, lipase, Mg, K, total cholesterol, UA 벑 9빆紐⑹씠 V-Tube Greiner-Tube 鍮꾧탳뿉꽌뒗 AST, direct bilirubin, HDL-C, LDL-C, Mg, K, TSH쓽 7빆紐⑹씠 쑀쓽븳 寃곌낵 李⑥씠(P<0.05)瑜 蹂댁떎. 洹몃━怨 BD-Tube Greiner-Tube뿉꽌뒗 珥 11빆紐⑹씠 쑀쓽븳 寃곌낵 李⑥씠瑜 蹂댁떎. 洹몃윭굹 씠뱾 紐⑤몢 媛뺥븳 뼇쓽 꽑삎 긽愿愿怨꾨 蹂댁쑝硫 엫긽쟻쑝濡 쓽誘 엳뒗 李⑥씠뒗 븘땲뿀떎. 吏吏덇궗씤 total cholesterol씠굹 LDL-C, HDL-C 빆紐⑹뿉꽌 媛 梨꾪삁愿 媛 쑀쓽븳 李⑥씠瑜 蹂댁뒗뜲, 듅엳 LDL-C 빆紐⑹뿉꽌 Greiner-Tube쓽 痢≪젙媛(mean±standard deviation [SD], 122.23±46.04)씠 V-Tube (mean±SD, 109.37±33.41) BD-Tube (mean±SD, 109.58±33.39)쓽 寃곌낵 빟 10% 李⑥씠瑜 蹂댁떎. 씠 李⑥씠뒗 Bland-Altman plot뿉꽌 븣 닔 엳벏씠 빟 125 mg/dL 씠긽쓽 깦뵆쓣 痢≪젙븷 븣 諛쒖깮븯뒗 寃쏀뼢씠 엳뿀떎. 씠뒗 媛 젣議곗궗蹂 寃곌낵媛믪쓽 李⑥씠媛 CLIA’88쓽 엫긽쟻 뿀슜踰붿쐞瑜 留뚯”븯뒗 踰붿쐞 궡濡 옉吏留, 엫긽쟻 뿀슜 媛뒫媛믪쓣 踰쀬뼱굹 梨꾪삁愿 옄泥닿 寃궗寃곌낵뿉 誘몄튂뒗 슂씤씠 맆 닔룄 엳쓬쓣 蹂댁뿬二쇱뿀떎.

씠踰 뿰援щ뒗 3媛쒖쓽 쓽猷뚭린愿뿉꽌 李몄뿬븿쑝濡쒖뜥 寃궗쓽 寃곌낵媛믪씠 寃궗옣鍮꾩 떆빟, 洹몃━怨 궗슜옄 媛숈 寃궗떎솚寃쎌쓽 李⑥씠濡 씤븯뿬 쁺뼢쓣 諛쏆쓣 닔 엳쓣 寃껋쑝濡 깮媛곷릺뼱 梨꾪삁愿쓽 踰붿슜꽦쓣 룊媛빐 蹂 닔 엳쓣 寃껋쑝濡 湲곕븯떎. 媛 李몄뿬 쓽猷뚭린愿蹂 梨꾪삁愿 媛 寃곌낵媛믪쓽 李⑥씠뒗 CLIA’88쓽 뿀슜踰붿쐞瑜 留뚯”븯떎. 洹몃윭굹 TSH 빆紐⑹뿉꽌 Architect i2000sr analyzer 옣鍮꾨줈 痢≪젙븳 40媛 寃泥댁쓽 TSH 룊洹좉컪 Greiner-Tube (mean±SD, 1.38±0.98) 룊洹좉컪怨 V-Tube (mean±SD, 1.73±1.23) BD-Tube (mean±SD, 1.73±1.25)뿉 鍮꾪븯뿬 쑀쓽븳 李⑥씠瑜 蹂댁떎. DXI 800 옣鍮꾨줈 痢≪젙븳 60媛 寃泥댁쓽 TSH 룊洹좉컪 V-Tube (mean±SD, 2.14±2.60), BD-Tube (mean±SD, 2.08±2.41), Greiner-Tube (mean±SD, 2.13±2.63)濡 tube蹂 寃곌낵媛믪쓽 겙 李⑥씠뒗 蹂댁씠吏 븡븯떎. 씠윭븳 寃곌낵뒗 tube쓽 슂씤怨 洹 쇅 떎瑜 寃궗솚寃쎌쓽 슂씤씠 꽌濡 寃고빀븯뿬 寃궗寃곌낵뿉 쁺뼢쓣 以 닔 엳떎뒗 젏쓣 떆궗븳떎.

삁븸 寃泥대 씠슜븳 寃궗뒗 梨꾪삁 썑 씪젙븳 떆媛 궡뿉 寃궗媛 吏꾪뻾릺뼱빞 븯吏留 옱寃 벑怨 媛숈 씠쑀 븣臾몄뿉 떆媛꾩씠 寃쎄낵맂 썑뿉 떎떆 寃궗媛 떆뻾맆 븣룄 엳떎. 媛 寃궗빆紐⑸퀎 떆媛꾩쓽 吏뿰뿉 뵲瑜 寃궗쓽 寃곌낵移섏쓽 蹂솕뒗 留롮 臾명뿄뱾 넻븯뿬 삁긽븷 닔 엳쑝硫, 씠踰 뿰援ъ뿉꽌뒗 梨꾪삁 떦씪 2떆媛 씠궡, 梨꾪삁 썑 븯猷, 梨꾪삁 썑 3씪, 梨꾪삁 썑 7씪쓽 寃곌낵媛믪쓣 痢≪젙븯뿬 梨꾪삁愿 젣議곗궗蹂꾨줈 洹 蹂솕移섎 솗씤븯떎. 떆媛 寃쎄낵뿉 뵲瑜 蹂룞룺 3醫낅쪟쓽 梨꾪삁愿 紐⑤몢 CLIA’88瑜 留뚯”븯떎. 븯吏留 寃泥 梨꾩랬 썑 1씪 썑쓽 寃곌낵媛 媛옣 겙 룺쓽 蹂솕瑜 蹂댁씠誘濡 寃곌낵빐꽍 떆뿉 李멸퀬븷 닔 엳寃좊떎.

寃곕줎쟻쑝濡, 蹂 뿰援ъ뿉꽌뒗 엫긽寃궗떎뿉꽌 궗슜릺뒗 吏꾧났梨꾪삁愿쓽 젣議곗궗蹂, 蹂닿씪닔뿉 뵲씪 깮솕븰寃궗 諛 媛묒긽꽑寃궗뿉 쁺뼢쓣 以 닔 엳쓬쓣 솗씤븯쑝硫, 援궛 吏꾧났梨꾪삁愿씤 V-Tube媛 닔엯 梨꾪삁愿怨 룞벑븳 꽦뒫怨 븞젙꽦쓣 媛뽮퀬 엳쓬쓣 솗씤븯떎.

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