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Comparison of YD URiSCAN PluScope Urine Microscopic Analyzer and Sysmex UF-1000i Flow Cytometry Systems
J Lab Med Qual Assur 2018;40:223-229
Published online December 31, 2018
© 2018 Korean Association of External Quality Assessment Service.

Jae Won Jung, Ae-Chin Oh, Yoon Hwan Chang, Jin Kyung Lee, and Young Jun Hong

Department of Laboratory Medicine, Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences, Seoul, Korea
Correspondence to: Young Jun Hong
Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences, 75 Nowonro, Nowon-gu, Seoul 01812, Korea
Tel: +82-2-970-2492, Fax: +82-2-973-7143
E-mail: clinchem@kirams.re.kr
Received September 24, 2018; Revised October 19, 2018; Accepted October 24, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
Background: Urinalysis is one of the most commonly performed tests in clinical laboratories. In this study, we compared YD URiSCAN PluScope (PluScope; YD Diagnostics Corp., Korea) and Sysmex UF-1000i (UF-1000i; Sysmex Corp., Japan) for urine microscopic sediment analysis.
Methods: A total of 404 fresh urine samples were collected and analyzed using PluScope, UF-1000i, and manual microscopy. Quantitative correlation analyses for red blood cells (RBCs), white blood cells (WBCs), epithelial cells (EC), and casts were performed using Spearman셲 correlation. We evaluated agreement among the three systems by using weighted Cohen셲 觀 and calculating concordance rates within one grade of difference for semiquantitative and qualitative parameters.
Results: There were moderate-high correlations between PluScope and UF-1000i for RBCs, WBCs, and ECs (r =0.542, 0.714, and 0.571, respectively) but negligible correlation for casts (r =0.186). There were moderate-high correlations between manual microscopy and automated devices for RBCs, WBCs, and ECs (r =0.5500.745) but negligible correlations for casts (PluScope: r =0.247; UF-1000i: r =0.223). The pairwise concordance rates within one grade difference among the three methods were good for RBCs, WBCs, and ECs (95.0%99.0%, 觀=0.410.74). For casts, the concordance rate between PluScope and manual microscopy was fair (96.8%, 觀=0.25), but concordance rates between UF-1000i and manual microscopy and between the two automated devices were poor (81.2% and 81.7%; 觀=0.04 and 0.06, respectively).
Conclusions: The two automated urine sediment analyzers showed a moderate-high correlation and concordance rate. They showed good correlations and concordance rates for RBCs, WBCs, and ECs. However, manual microscopic examinations are still needed for reviewing and confirming the presence of pathologic particles in urine, such as casts and crystals.
Keywords : Automated urine sediment analyzer, Urinalysis, Urine sediment, URiSCAN PluScope, UF-1000i
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