Search for


TEXT SIZE

search for



CrossRef (0)
Inter-Laboratory Comparison of Multi-Allergen-Specific Immunoglobulin E Screening Assay Using Polycheck Allergy Diagnostic
J Lab Med Qual Assur 2019;41:39-46
Published online March 31, 2019
© 2019 Korean Association of External Quality Assessment Service.

Young Ae Lim

Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Korea
Correspondence to: Young Ae Lim Department of Laboratory Medicine, Ajou University School of Medicine, 164 World cup-ro, Yeongtong-gu, Suwon 16499, Korea Tel: +82-31-219-5786 Fax: +82-31-219-5778 E-mail: limyoung@ajou.ac.kr
Received November 21, 2018; Revised January 31, 2019; Accepted February 8, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract

Background:

Currently there is no external proficiency testing program for allergen-specific immunoglobulin E assays (sIgE) in Korea. The aim of this study was to evaluate the accuracy of sIgE performed in different laboratories in Korea using Polycheck Allergy Diagnostic (Biocheck GmbH, Germany) or simply 쁏olycheck and to further perform an inter-laboratory comparison of sIgE.

Methods:

Three inter-laboratory trials were undertaken from June 2016 to December 2017 at nine institutions. Serum samples obtained from three patients who had a history of positive Polycheck results were used in each trial. A total of 59 allergens were evaluated from the standard panel, inhalation panel, and food panel. The acceptable rates (%) of classes 06 of the results were calculated using three different criteria, including the consensus of 80% participants of the College of American Pathologists (CAP), the most frequent value (MFV), and the Overall Misclassification Index Score (OMIS).

Results:

The proportion of laboratories that showed acceptable results for CAP criteria, MFV, and OMIS were around 93.0%97.7%, 66.3%76.0%, and 0.11.3, respectively, in each trial. Under-performing institutes showed consistently higher (49/68, 72.1%) or lower (19/68, 27.9%) than acceptable results for all criteria.

Conclusions:

This is the first study evaluating the inter-laboratory variation of sIgE in Korea using various criteria. It was found that the acceptable rate could be altered based on changes in these criteria. These findings might be useful for the inter-laboratory comparison of sIgE in Korea in the future as well.

Keywords : Laboratory proficiency testing, Allergen-specific immunoglobulin E, Quality control
꽌 濡

븣젅瑜닿린 吏꾨떒 떆 썝씤씠 릺뒗 븣젅瑜닿쾺쓣 寃異쒗븯뒗 寃껋 以묒슂븯떎. 怨좎쟾쟻씤 吏꾨떒諛⑸쾿쑝濡 븣젮吏 뵾遺떒옄떆뿕 寃궗諛⑸쾿 諛 寃곌낵빐꽍쓽 몴以솕媛 뼱졄怨 빆엳뒪誘쇱젣 벑쓽 빟臾쇱쓣 蹂듭슜븯嫄곕굹 뵾遺吏덊솚씠 엳뒗 솚옄뿉寃뚮뒗 寃궗瑜 떎떆븷 닔 뾾떎뒗 떒젏씠 엳떎[1]. 씠뿉 鍮꾪븯뿬 삁泥 븣젅瑜닿쾺 듅씠 immunoglobulin E (IgE) 빆泥 痢≪젙踰뺤 媛꾪렪븯硫댁꽌룄 뵾遺떒옄떆뿕쓽 떒젏쓣 蹂댁셿븷 닔 엳떎뒗 옣젏쓣 媛吏怨 엳뼱 쁽옱 꽑蹂꾧궗濡 꼸由 궗슜릺怨 엳떎. 寃궗踰뺣룄 諛⑹궗꽑硫댁뿭痢≪젙踰뺤뿉꽌 理쒓렐뿉뒗 鍮꾧탳쟻 떎猷④린 렪由ы븳 슚냼硫댁뿭痢≪젙踰 샊 솕븰諛쒓킅硫댁뿭遺꾩꽍踰뺤쑝濡 移섑솚븯寃 릺뼱 뜑슧 寃궗媛 렪由ы븯寃 릺뿀떎.

삁泥 븣젅瑜닿쾺 듅씠 IgE 빆泥 痢≪젙踰뺤 떆빟쓽 븣젅瑜닿쾺 援ъ꽦뿉 뵲씪 겕寃 몢 媛吏濡 굹돖 닔 엳뒗뜲, 븣젅瑜닿쾺留덈떎 媛쒕퀎濡 媛쒕컻릺뼱 쓽떖릺뒗 듅젙 븣젅瑜닿쾺 듅씠 IgE 빆泥댁쓽 寃異쒖쓣 쐞븳 媛쒕퀎寃궗 떎以 븣젅瑜닿쾺 뙣꼸쓣 씠슜븯뒗 꽑蹂꾩슜 뙣꼸寃궗씠떎. 씠 以 Polycheck Allergy Diagnostic (Polycheck; Biocheck GmbH, M체nster, Germany)뒗 떎以 븣젅瑜닿쾺 꽑蹂꾩슜 뙣꼸寃궗濡 援궡뿉꽌 씠誘 떎瑜 꽑蹂꾧궗뿉 鍮꾪븯뿬 룞벑븯嫄곕굹 슦닔븳 寃껋쑝濡 룊媛릺뿀떎[2,3]. Polycheck뒗 媛곴컖 20媛쒖쓽 븣젅瑜닿쾺쑝濡 援ъ꽦맂 移댁꽭듃 삎깭濡 珥 IgE瑜 룷븿븳 븳援삎 I 몴以삎(몴以삎), 븳援삎 II 씉씤 꽑蹂꾩슜(씉엯슜), 븳援삎 III 떇뭹 꽑蹂꾩슜(떇뭹삎)쓽 3媛吏 移댁뀑듃 삎깭濡 援ъ꽦릺뼱 珥 IgE瑜 룷븿븯뿬 60媛吏쓽 븣젅瑜닿쾺쓣 꽑蹂꾪븷 닔 엳떎.

븣젅瑜닿쾺 듅씠 IgE 빆泥 떆빟뱾 젣議고쉶궗留덈떎 떆빟쓽 븣젅瑜닿린빆썝 꽦遺꾩씠 떎瑜닿퀬, 젙웾 蹂댁젙諛⑸쾿, 寃異쒕갑踰뺤씠 떎瑜대濡 븣젅瑜닿쾺 듅씠 IgE 빆泥 寃곌낵媛 떆빟 媛꾩뿉 떎瑜 닔 엳湲 븣臾몄뿉[3] 떎瑜 떆빟뱾 媛꾩쓽 寃곌낵瑜 鍮꾧탳븷 寃쎌슦뿉뒗 二쇱쓽븯뿬빞 븳떎. 삉븳 꽭怨꾨낫嫄닿린援(World Health Organization, WHO) 궛븯쓽 깮臾쇳븰쟻몴以솕쟾臾몄쐞썝쉶(Expert Committee on Biological Standardization)뿉꽌 씪遺 븣젅瑜닿쾺뿉 븳 WHO international biological reference preparations瑜 젣議고븯湲곕뒗 븯굹[4], 씠쓽 醫낅쪟媛 썙굺 떎뼇븯怨 빆썝異붿텧臾쇱쓣 궗슜븯뒗 寃궗踰뺤쓽 듅꽦 븣臾몄뿉 븣젅瑜닿쾺 듅씠 IgE 빆泥댁쓽 몴以솕 옉뾽 돺吏 븡 떎젙씠떎. 뵲씪꽌 媛 寃궗떎뿉꽌뒗 떆빟젣議고쉶궗뿉꽌 젣怨듯븯뒗 calibrator瑜 궗슜븯뿬 二쇨린쟻씤 蹂댁젙쓣 븯怨 궡遺 젙룄愿由ъ 쇅遺닕젴룄議곗궗瑜 넻븯뿬 寃곌낵쓽 젙솗꽦쓣 쑀吏븯룄濡 沅뚭퀬븯怨 엳떎.

洹몃읆뿉룄 遺덇뎄븯怨 떆빟 媛꾩뿉 떎뼇븳 寃곌낵瑜 蹂댁씠湲 븣臾몄뿉 쁽옱 援궡뿉꽌 寃泥 寃궗뿉 븯뿬 쇅遺닕젴룄議곗궗궗뾽쓣 떞떦븯뒗 吏꾨떒寃궗쓽븰쉶 븳엫긽寃궗젙룄愿由ы삊쉶뿉꽌뒗 븣젅瑜닿쾺 듅씠 IgE 빆泥댁뿉 븳 쇅遺닕젴룄議곗궗媛 븘吏 뾾뒗 떎젙씠떎. 떎留 듅젙 援궡 떆빟젣뭹뿉 븳븯뿬 洹 젣뭹쓣 궗슜븯怨 엳뒗 援궡 寃궗떎뿉 븳븯뿬 쇅遺닕젴룄議곗궗媛 씪遺 吏꾪뻾릺怨 엳뒗 寃껋쓣 젣쇅븯怨좊뒗 援궡 쇅遺닕젴룄議곗궗궗뾽씠 吏꾪뻾릺怨 엳吏 븡븘 寃궗떎 媛 鍮꾧탳 룊媛媛 뼱젮슫 떎젙씠떎. 臾쇰줎 씪遺 삎蹂묒썝뿉꽌뒗 援궡媛 븘땶 쇅援뿉꽌 二쇨븯怨 엳뒗 븣젅瑜닿쾺 듅씠 IgE 빆泥댁뿉 븳 쇅遺닕젴룄議곗궗뿉 李몄뿬븯怨 엳쑝굹 留롮 援궡 湲곌뿉꽌뒗 李몄뿬븯怨 엳吏 紐삵븯뒗 떎젙씠떎.

씠뿉 蹂 뿰援ъ뿉꽌뒗 援궡뿉꽌 Polycheck 젣뭹쓣 怨듦툒븯뒗 二쇱떇쉶궗 썝硫붾뵒移쇱쓽 슂泥뿉 쓽븯뿬 쇅遺닕젴룄議곗궗 紐⑹쟻쑝濡 寃궗떎 媛 鍮꾧탳 룊媛슜 臾쇱쭏쓣 젣議고븯怨 援궡뿉 Polycheck Allergy Diagnostic 젣뭹쓣 궗슜븯怨 엳뒗 蹂묒썝뿉 諛쒖넚븯怨 洹 寃곌낵瑜 떎븰뼌 湲곗뱾濡 遺꾩꽍븯湲곗뿉 뼢썑 븣젅瑜닿쾺 듅씠 IgE 빆泥 寃궗쓽 寃궗떎 媛 룊媛 떆 湲곗 꽑젙뿉 룄쓣 二쇨퀬옄 븯떎.

옱猷 諛 諛⑸쾿

1. 긽

씠 뿰援щ뒗 寃泥 怨듭뿬옄 꽑젙쓣 쐞븯뿬 븘二쇰븰援먮퀝썝 깮紐낆쑄由ъ떖쓽쐞썝쉶쓽 듅씤쓣 뱷븳 썑 吏꾪뻾븯떎(AJIRB-DEV-SUR-16-156). 鍮꾧탳룊媛슜 臾쇱쭏 젣議곕 쐞븳 寃泥 怨듭뿬옄 꽑젙湲곗 吏꾨즺瑜 쐞븯뿬 넻긽寃궗濡쒖꽌 Polycheck 떎以 븣젅瑜닿쾺 듅씠 IgE 빆泥 寃궗媛 쓽猶곕맂 寃泥 以 떎以 븣젅瑜닿쾺뿉 뼇꽦쓣 蹂댁뿬 寃궗떎 媛 鍮꾧탳 紐⑹쟻쓣 쐞븳 臾쇱쭏젣議곗뿉 쟻빀븳 寃쎌슦씪怨 뿰援ъ옄媛 뙋떒븳 寃쎌슦濡 븳젙븯떎. 留 19꽭 씠긽쓽 꽦씤쑝濡 援궡 400 mL 쟾삁 뿄삁옄 湲곗쓣 異⑹”븯硫 泥대궡 삁븸쓽 2% 誘몃쭔씤 66 mL瑜 梨꾪삁븯뿬룄 臾대━媛 뾾쓣 寃껋쑝濡 뿬寃⑥뒗 쟾떊긽깭 뼇샇븳 꽦씤쑝濡 븯떎. 씠뱾 以 寃泥 젣怨듭뿉 룞쓽븯뿬 꽕紐낅Ц 諛 룞쓽꽌뿉 꽌紐낇븳 뵾뿕옄뿉 븳븯뿬 쟾삁쓣 梨꾪삁븯떎. 珥 3쉶 諛쒖넚쓣 紐⑺몴濡 諛쒖넚 떆留덈떎 븳 紐낆쓽 뵾뿕옄濡쒕꽣 쑀옒맂 寃泥대 梨꾪삁븯뿬 젣議고븯떎.

寃궗떎 媛 鍮꾧탳 룊媛뿉 李몄뿬븷 湲곌 Polycheck 젣뭹쓣 怨듦툒븯뒗 二쇱떇쉶궗 썝硫붾뵒移쇱뿉꽌 媛 湲곌뿉 븞궡瑜 븯뿬 李몄뿬쓽궗瑜 諛앺엺 湲곌뱾濡 븳젙븯떎. 빆紐⑹ 珥 IgE瑜 룷븿븯뿬 Polycheck 젣뭹쓽 몴以삎 20媛, 씉엯삎 20媛, 떇뭹삎 20媛쒕줈 珥 IgE瑜 룷븿븳 60媛 븣젅瑜닿쾺씠 룷븿맂 3媛吏 醫낅쪟쓽 뒪듃由쎌쓣 룊媛븯떎.

2. 뿰援ъ꽕怨

1) 寃궗떎 媛 鍮꾧탳 룊媛슜 臾쇱쭏 젣怨듭옄 꽑젙

뿰援ъ옄媛 뵾뿕옄 議곌굔뿉 쟻빀븳 씠뱾씠씪怨 뙋떒릺硫 넻긽 寃궗瑜 쓽猶고븳 엫긽쓽궗뱾뿉寃 뿰씫쓣 痍⑦븯뿬 뵾뿕옄瑜 寃궗떎뿉 諛⑸Ц븯寃 븯뿬, 뿰援 李몄뿬뿉 븳 꽕紐낆쓣 뱽怨 룞쓽瑜 븳 옄뿉 븳븯뿬 梨꾪삁쓣 떎떆븯떎. 梨꾪삁맂 寃泥대뒗 썝떖遺꾨━ 썑 삁泥쓣 뿬윭 媛쒕줈 遺꾩<븯뿬 몴떇븳 썑 70째C뿉 蹂닿븯떎.

2) 寃궗떎 媛 鍮꾧탳 룊媛슜 臾쇱쭏 젣議 諛 怨듦툒

룊媛 떆留덈떎 씠誘 蹂닿맂 삁泥쓣 끃뿬 媛 떆뿕愿뿉 遺꾩<(1-1.5 mL)븯怨, 씡紐낇솕맂 슜湲 몴떇, 寃닔 諛 븘씠뒪뙥 룷옣옉뾽쓣 떆뻾븳 썑 슦泥닿뎅 씡씪 벑湲 諛곗넚 떆源뚯 깋옣蹂닿븳 썑 寃泥대 諛곗넚븯떎. 寃泥 諛쒖넚 1二쇱씪 쟾뿉 李몄뿬湲곌뿉 븞궡臾몄쓣 癒쇱 諛쒖넚븯뿬 븞궡瑜 떎떆븯떎.

3) 寃궗떎 媛 鍮꾧탳 룊媛寃곌낵 遺꾩꽍 諛 諛고룷

寃泥 諛쒖넚 3二쇱씪 씠궡濡 떟蹂쓣 쉶떊諛쏄퀬 2떖 씠궡濡 遺꾩꽍븯뿬 寃곌낵瑜 李몄뿬湲곌쓽 硫붿씪濡 쉶떊븯떎. 李몄뿬湲곌쓽 쉶떊寃곌낵瑜 諛뷀깢쑝濡 珥 IgE瑜 젣쇅븳 59媛 븣젅瑜닿쾺뿉 븳 媛 빆紐⑹쓣 벑湲(class) 0遺꽣 6源뚯쓽 寃곌낵瑜 遺꾩꽍븯떎. 遺쟻빀 뿀슜踰붿쐞 씠쇅쓽 寃곌낵瑜 蹂댁씠뒗 벑湲됱쑝濡 젙쓽븯쑝硫, 媛 湲곌蹂 李몄뿬湲곌쓽 寃곌낵뒗 씡紐낆쑝濡 씠硫붿씪濡 遺꾩꽍寃곌낵瑜 諛쒖넚븯떎. 벑湲됱쓽 뿀슜踰붿쐞뒗 쉶李 떆留덈떎 씪遺 李⑥씠媛 엳뿀吏留 蹂 뿰援щ 쐞븳 理쒖쥌 遺꾩꽍 떆뒗 뿀슜踰붿쐞瑜 넻씪븯湲 쐞븯뿬 3媛吏 湲곗쑝濡 룊媛븯떎. 誘멸뎅蹂묐━삊쉶(College of American Pathologists, CAP)쓽 Multiple Allergen Screen쓽 2018뀈 湲곗泥섎읆 李몄뿬湲곌쓽 80% 빀쓽(consensus) 씠긽, 1997뀈 쑀읇 쇅遺닕젴룄룊媛 蹂닿퀬뿉꽌 궗슜븳 Overall Misclassification Index Score (OMIS) [5], 洹몃━怨 삤뒪듃由ъ븘쓽 25뀈媛꾩쓽 쇅遺닕젴룄룊媛 寃곌낵 遺꾩꽍뿉꽌 궗슜맂 Koch Aberer [6]쓽 湲곗쓣 궗슜븯떎. 鍮꾨줉 CAP뿉꽌뒗 李몄뿬湲곌씠 10媛 씠긽씤 寃쎌슦뿉 넻怨꾨텇꽍쓣 떎떆븯怨 5媛 씠긽씤 寃쎌슦뿉뒗 以묒븰媛, 理쒓퀬 諛 理쒖媛믩쭔쓣 굹궡怨 엳쑝굹 蹂 뿰援ъ뿉꽌뒗 李몄뿬湲곌씠 紐⑤몢 10湲곌 誘몃쭔씠뿀쑝굹 룊媛瑜 쐞븯뿬 80% 빀쓽 씠긽쓣 湲곗쑝濡 궪븯떎. CAP 湲곗쓽 쟻빀瑜좎 留 쉶李 59媛 빆썝쓣 遺꾨え濡 쟻빀븳 빆썝 닔瑜 遺꾩옄濡 븯뿬 궛젙븯떎. Misclassification Index Score (MIS) 吏닔뒗 媛 빆썝留덈떎 벑湲 0怨 1 쓬꽦씠怨 洹 씠긽쓽 벑湲됱 뼇꽦쑝濡 媛꾩<븯怨 李몄뿬湲곌쓽 80% 씠긽쓽(썝옒뒗 90% 씠긽씠굹 蹂 뿰援щ뒗 李몄뿬湲곌 닔媛 쟻쑝誘濡 90% 씠긽씠젮硫 紐⑤뱺 湲곌쓽 떟蹂씠 씪移섑븯뿬빞 븯誘濡 닔젙븿) 빀쓽瑜 뼸 쓬꽦 샊 뼇꽦 寃곌낵瑜 理쒖쥌 젙떟쑝濡 븯릺 젙떟湲곌 0젏, 삤떟湲곌 1젏쓣 二쇰뒗 諛⑹떇쑝濡 紐⑤뱺 빆썝쓽 MIS 媛믪쓽 빀쓣 OMIS濡 젙쓽븯떎. Koch Aberer [6]쓽 湲곗 理쒕퉰룄 媛(most frequent value)쓣 뿀슜踰붿쐞濡 젙쓽븯릺, 理쒕퉰룄짹1벑湲 샊 2벑湲됯퉴吏룄 媛뒫븳 뿀슜踰붿쐞濡 遺꾩꽍븯떎. 삉븳 뼇꽦 寃泥댁쓽 寃쎌슦 젅 젙떟뿉꽌 2벑湲 珥덇낵씠嫄곕굹 쓬꽦쑝濡 떟蹂븳 寃쎌슦 쓬꽦 寃泥댁쓽 寃쎌슦 뼇꽦쑝濡 蹂닿퀬븳 寃쎌슦瑜 遺쟻빀 寃곌낵濡 젙쓽븯뿬 遺쟻빀瑜좎쓣 궛젙븯떎.

2李⑥ 3李 룊媛뿉꽌뒗 寃궗떎 媛 李⑥씠瑜 蹂댁씠嫄곕굹 씪遺 솗씤씠 븘슂븯뜕 븣젅瑜닿쾺 듅씠 IgE 빆泥 빆紐 以 Im異럘noCAP (Pharmacia Diagnostics AB, Uppsala, Sweden)쑝濡 솗씤씠 媛뒫븯뜕 빆紐⑹뿉 빐꽌뒗 ImmunoCAP 寃궗瑜 떆뻾븯떎. 씠 寃쎌슦 ImmunoCAP 寃곌낵源뚯 李몄뿬湲곌뿉 怨듭븯릺, Polycheck쓽 f24/440 (Shrimp Mix)뒗 ImmunoCAP뿉꽌뒗 f24쑝濡 빆썝 議곗꽦씠 씪遺 떎由꾩쑝濡 寃곌낵媛믪씠 떎瑜 닔 엳쓬쓣 븿猿 怨듭븯떎.

4) 넻怨꾨갑踰

옄猷뚮뒗 Excel professional plus 2010 봽濡쒓렇옩(Microsoft Corp., Redmond, WA, USA)濡 젙由ы븯怨, 넻怨꾨텇꽍 SPSS ver. 12.0 for Windows (SPSS Inc., Chicago, IL, USA)쓣 궗슜븯뿬 遺꾩꽍븯떎. 3쉶쓽 룊媛 룞븞 李몄뿬븳 7湲곌뱾쓽 쉶李 媛 寃곌낵 鍮꾧탳뒗 Friedman Test濡 寃利앺븯怨, 媛 湲곗룊媛諛⑸쾿뿉 뵲瑜 3쉶李 룞븞쓽 紐⑤뱺 젙떟瑜좎쓽 긽愿愿怨꾨뒗 Pearson correlation쑝濡 遺꾩꽍븯떎. P<0.05씤 寃쎌슦 쑀쓽븳 李⑥씠媛 엳뒗 寃껋쑝濡 媛꾩<븯떎.

5) 룊媛 썑 議곗튂

紐⑤뱺 李몄뿬湲곌뱾 젙湲곗쟻쑝濡 떆빟 怨듦툒뾽泥대줈遺꽣 젙湲곗젏寃쓣 諛쏆븯쑝굹, 留 쉶李 룊媛 썑 1媛 씠긽쓽 빆紐⑹뿉꽌 뿀슜踰붿쐞瑜 踰쀬뼱궃 寃곌낵瑜 蹂댁씤 湲곌뿉 빐꽌뒗 異붽濡 떆빟 怨듦툒뾽泥닿 湲곌린젏寃쓣 떎떆븯怨 젏寃 썑 긽뭹솕 議곕Ъ吏덈줈(Control-Sample for I-Standard Panel, Biocheck GmbH) 솗씤쓣 떎떆븯떎. 湲곌린 젏寃 떆뒗 뒠鍮숈뿉 븳 꽭泥(븘슂 떆 援먰솚), 移대찓씪 紐⑤뱢, x/y異뺢낵 紐⑤뱢뿉 븳 젏寃(겕由щ떇, 븘슂 떆뒗 援먯껜 諛 湲곕쫫 移 벑), 移쇰━釉뚮젅씠뀡 벑 떎떆, 럩봽뿉 븳 젏寃, 똻 援먯껜 벑씠 룷븿릺뿀떎.

3. 寃궗諛⑸쾿

1) Polycheck 寃궗

諛⑸쾿 쟾슜 옄룞솕 옣鍮꾨 씠슜븯뿬 떆빟꽕紐낆꽌뿉 以븯怨, 媛꾨왂엳 냼媛쒗븯硫 떎쓬怨 媛숇떎. 듅씠 븣젅瑜닿쾺씠 遺李⑸맂 移댁꽭듃쓽 nitrocellulose 留 쐞뿉 삁泥 200 uL쓣 遺꾩<, 빆-IgE瑜 遺꾩<, 슚냼 몴吏 젒빀泥대 꽔뿀뒗뜲, 媛 떒怨꾨쭏떎 諛섏쓳 썑 꽭泥숉븯떎. 뿬湲곗뿉 諛쒓킅떆빟쓣 꽔쑝硫 寃泥 궡 듅씠 IgE쓽 냽룄뿉 鍮꾨븯뿬 諛쒖깋븯뒗뜲, 諛쒖깋 媛뺣룄뒗 뒪罹먮꼫濡 뙋룆릺뼱 5媛쒖쓽 calibrator 寃곌낵媛믪뿉 洹쇨굅븯뿬 Biocheck Image Software 봽濡쒓렇옩씠 뒪罹뷀븳 씠誘몄瑜 젙웾 遺꾩꽍븯떎. 寃곌낵移섎뒗 kU/L濡 굹궡뿀쑝硫, >100 kU/L 100 kU/L쑝濡 媛꾩<븯떎. 珥 냼슂떆媛꾩 2떆媛 40遺꾩씠뿀쑝硫 젙웾 寃곌낵媛믨낵 벑湲됱쓣 0-6쓣 궛異쒗븯뒗뜲, 젙웾媛믪 0.35 kU/L 씠긽, 벑湲 1 씠긽쓣 뼇꽦쑝濡 뙋젙븯떎.

2) ImmunoCAP

諛⑸쾿 쟾슜옣鍮꾩씤 ImmunoCAP250 (Pharmacia Diagnostics AB)怨 諛 븣젅瑜닿쾺 듅씠 IgE 쟾슜떆빟쓣 씠슜븯뿬 떆빟꽕紐낆꽌뿉 以븯뿬 寃궗瑜 떆뻾븯떎. 利 삁泥 40 uL瑜 븣젅瑜닿쾺씠 寃고빀릺뼱 엳뒗 ImmunoCAP 罹≪뒓怨 諛섏쓳 썑 꽭泥, 슚냼 몴吏 빆-IgE瑜 꽔怨 諛섏쓳 썑 꽭泥숉븯怨, 삎愿묐컻깋떆빟쓣 꽔뼱 諛섏쓳떆궓 썑 삎愿 젙룄瑜 냽룄濡 쟾솚븯뿬 젙웾 遺꾩꽍븯떎. 寃곌낵移섎뒗 kU/L濡 굹궡뿀쑝硫, >100 kU/L 100 kU/L쑝濡 媛꾩<븯떎. 珥 냼슂떆媛꾩 빟 2떆媛꾩씠硫 젙웾 寃곌낵媛믨낵 벑湲됱쓣 0-6쓣 궛異쒗븯뒗뜲, 젙웾媛믪 0.35 kU/L 씠긽, 벑湲 1 씠긽쓣 뼇꽦쑝濡 뙋젙븯떎.

寃 怨

寃궗떎 媛 鍮꾧탳 룊媛媛 씠猷⑥뼱吏 李몄뿬湲곌 닔뒗 珥 9媛쒕줈 3쉶 紐⑤몢 李몄꽍븳 湲곌 7媛쒖쑝硫 떆湲곕뒗 떎쓬怨 媛숇떎. 2016뀈 11썡 9湲곌, 2017뀈 5썡 8湲곌, 2017뀈 10썡 7湲곌 珥 3李⑤뿉 嫄몄퀜 떆뻾븯떎. 뵾뿕옄뒗 臾쇱쭏젣議곗뿉 쟻빀븯떎怨 솗씤맂 3紐낆쓽 꽦씤쓣 꽑젙븯뿬 媛 쉶李⑤퀎 룊媛떦 1紐낆쓽 뵾뿕옄 삁븸쓣 궗슜븯떎. 李몄뿬븳 紐⑤뱺 湲곌씠 씉엯삎, 떇뭹삎, 洹몃━怨 몴以삎뿉 李몄뿬븯쑝굹, 3쉶뿉 李몄뿬븳 湲곌 以 븳 湲곌 떇뭹삎怨 몴以삎뿉留 李몄뿬븯떎.

媛 쉶李⑤퀎 59媛 븣젅瑜닿쾺 빆썝 룊媛뿉꽌 CAP 湲곗 쟻슜 떆 젙떟瑜좎쓽 룊洹좎 1李 94.7%, 2李 97.7%, 洹몃━怨 3李 93.0%쑝굹 3쉶 紐⑤몢 李몄뿬븳 7湲곌뱾쓽 쉶李⑤퀎 젙떟瑜좎쓽 李⑥씠뒗 뾾뿀떎(P=0.53). Koch Aberer [6]쓽 理쒕퉰룄 媛믩쭔쓣 뿀슜湲곗쑝濡 쟻슜 떆 젙떟瑜좉낵 遺쟻빀瑜좎쓽 룊洹좎 媛곴컖 1李 76.0% 0.9%, 2李 75.4% 0.6% 洹몃━怨 3李 66.3% 6.5%쑝굹 3쉶 紐⑤몢 李몄뿬븳 7湲곌뱾쓽 쉶李⑤퀎 젙떟瑜좎쓽 李⑥씠뒗 뾾뿀떎(P=0.65). OMIS 吏닔쓽 룊洹좎 1李 0.2, 2李 0.1, 洹몃━怨 3李 1.3쑝濡 利앷븯쑝굹 3쉶 紐⑤몢 李몄뿬븳 7湲곌뱾쓽 쉶李⑤퀎 OMIS 吏닔쓽 李⑥씠뒗 뾾뿀떎(P=0.37) (Table 1).

Table 1 . Percentage (%) of allergens from participating laboratories showing unacceptable results using Polycheck Allergy Diagnostic during three trials.

TrialLaboratoryCAP (2018)Most frequent value (2014)OMIS (1997)


%Result*Frequency짹1 class짹2 classFalse
1A100.066.789.7100.00.00
2100.089.7100.0100.00.00
3100.046.264.194.95.10
1B96.6Lower76.398.3100.00.00
2100.083.198.3100.00.00
3100.093.298.3100.00.00
1C98.3Higher79.791.596.63.40
293.2Lower71.284.7100.00.02
394.9Lower78.094.9100.00.00
1D96.6Lower76.394.9100.00.00
2100.086.4100.0100.00.00
3100.081.4100.0100.00.00
1E89.8Lower76.389.8100.00.00
296.6Higher71.289.896.63.40
398.3Lower66.189.8100.00.00
1F100.067.886.498.31.71
293.2Higher78.094.9100.00.00
1G100.072.9100.0100.00.00
298.3Higher78.094.998.31.71
357.6Higher27.139.078.022.04
1H72.9Higher45.869.593.222.04
1I98.3Lower83.1100.0100.00.00
2100.076.389.8100.00.00
3100.081.494.9100.00.00
1Total94.776.091.199.10.90.2
297.775.493.299.40.60.1
393.066.384.095.66.51.3

CAP, consensus of 80% participants from the College of American Pathologists; most frequent value, using criteria based on the results of 25 years of external quality assessment in Austria [6]; and OMIS, using criteria from external proficiency testing carried out in Europe in 1997 [5]..

Abbreviations: CAP, College of American Pathologists; OMIS, Overall Misclassification Index Score..

*All grades of unacceptable results from under-performing institutes were consistently higher or lower as compared to the acceptable results obtained from compliant laboratories.


CAP 湲곗쓣 쟻슜븷 寃쎌슦 媛 쉶떦 삤瑜섎 蹂댁씤 媛 븣젅瑜닿쾺 빆紐⑹쓽 媛쒖닔(湲곌 닔)뒗 1 (4), 2 (3), 3 (1), 4 (2), 6 (1), 16 (1), 25 (1)쑝濡 뿀슜踰붿쐞瑜 踰쀬뼱궃 寃쎌슦媛 68媛쒖뒗뜲, 씠 以 41媛쒕뒗 2媛 湲곌쑝濡쒕꽣 쑀옒릺뿀떎. 遺쟻빀 寃곌낵瑜 蹂댁씤 紐⑤뱺 湲곌뿉꽌 遺쟻빀쓣 蹂댁씤 뼇긽 떎瑜 湲곌뿉 鍮꾪븯뿬 씪愿꾩쟻쑝濡 쐞뼇꽦 샊 쐞쓬꽦 뼇긽쓣 蹂댁뿬二쇱뿀뒗뜲, 쐞뼇꽦씠 72.1% (49/68) 洹몃━怨 쐞쓬꽦씠 27.9% (19/68)瑜 李⑥븯뿬 쐞뼇꽦씠 뜑 留롮븯떎(Table 1).

3쉶李 룞븞쓽 紐⑤뱺 젙떟瑜좎쓽 Pearson 긽愿怨꾩닔뒗 CAP 湲곗怨 Koch Aberer [6]쓽 理쒕퉰룄 湲곗 쟻슜 떆뒗 0.759 (P<0.001), CAP 湲곗怨 Koch Aberer [6]쓽 理쒕퉰룄짹1 벑湲됯린以 쟻슜 떆뒗 0.798 (P<0.001), CAP 湲곗怨 Koch Aberer [6]쓽 理쒕퉰룄짹2 벑湲됯린以 쟻슜 떆뒗 0.641 (P<0.001) 洹몃━怨 Koch Aberer [6] 湲곗쓽 遺쟻빀瑜좉낵 OMIS 吏닔쓽 긽愿怨꾩닔뒗 0.894 (P<0.001)濡 媛옣 넂븯떎.

諛섎㈃ 珥 IgE瑜 룷븿븳 59媛 븣젅瑜닿린 빆썝 以 遺쟻빀쓣 蹂댁씤 빆썝 以묐났 빆紐⑹쓣 젣쇅븯怨좊뒗 43媛 빆紐⑹씠뿀떎. 59媛 빆썝 以 CAP 湲곗쑝濡 3쉶李 紐⑤몢뿉꽌 遺쟻빀 寃곌낵瑜 蹂댁씤 湲곌씠 엳嫄곕굹 3쉶李 以 븳踰덉씠씪룄 Koch Aberer [6]쓽 湲곗뿉 쓽븯뿬 3벑湲 씠긽쓣 踰쀬뼱궃 湲곌씠 엳嫄곕굹 샊 몢 湲곗뿉꽌 2쉶李 씠긽 遺쟻빀 寃곌낵瑜 蹂댁씠硫댁꽌 3쉶李 以 븳踰덉씠씪룄 Koch Aberer [6]쓽 湲곗뿉 쓽븯뿬 2벑湲 씠긽쓣 踰쀬뼱궃 빆썝뱾쓣 議곗궗빐 蹂댁븯뒗뜲 떎쓬怨 媛숈븯떎. 몴以삎뿉뒗 f14 (Soybean), e02/e05 (dog epithelia), d01 (Dermatophagoides pteronyssinus), w01 (ragweed pollen) g06 (timothy grass pollen), 쓬떇삎뿉뒗 f04 (wheat-flour), f11 (buckwheat-flour), f13 (peanut), f25 (tomato), f47 (garlic) citrus 샎빀臾, 씉엯삎뿉뒗 w08 (dandallon pollen), w12 (goldenrod pollen), k82 (latex), g03 (cocksfoot pollen), g04 (fescue meadow pol), g09 (redtop pollen), t04 (hazel pollen), w14 (pigweed) 洹몃━怨 Ash (ash mix)濡 쓬떇삎蹂대떎뒗 씉엯삎 빆썝씠 뜑 留롮븯떎.

ImmunoCAP 寃궗뒗 以묐났쓣 룷븿븯뿬 20媛 빆썝뿉꽌 떆뻾븯뒗뜲 17媛쒓 Polycheck쓽 뿀슜踰붿쐞 ImmunoCAP쓽 class 媛믪씠 씪移섑븯뿬 85%쓽 씪移섏쑉쓣 蹂댁怨, 洹 以 13媛쒕뒗 Polycheck쓽 뿀슜踰붿쐞 以 理쒕퉰룄 媛믨낵 씪移섑븯떎. 洹몃윭굹 ImmunoCAP쓽 class 媛믪씠 씪移섑븯쑝굹 f24/440 (shrimp mix), 2쉶李⑥쓽 g03 (Cocksfoot pollen), 3쉶李⑥쓽 f13 (peanut) 李몄뿬湲곌쓽 뿀슜踰붿쐞 ImmunoCAP쓽 class 媛믪씠 씪移섑븯吏 븡븯뒗뜲, 븳 떒怨 젙룄쓽 李⑥씠瑜 蹂댁떎(Table 2).

Table 2 . Comparison of class results of Polycheck Allergy Diagnostic and ImmunoCAP.

PanelCodeItemNo. of trialNo. of participantsClass of Polycheck (no. of participants)ImmunoCAP


AcceptableUnacceptableClasskUA/L
S긢24/440Shrimp mix370 (6)2 (1)10.51
S긢95Peach280 (2), 1 (2), 2 (4)(0)21.31
S긢95Peach373 (3), 4 (2), 6 (2)(0)311.3
S긠01Dermatophagoides pteronyssinus373 (5), 5 (1)6 (1)34.38
S긡01Cat epithelia373 (2), 4 (3), 5 (1)6 (1)35.86
S긲02/t03Alder-Birch pollen mix282 (8)(0)20.77
S긶01Ragweed pollen373 (4), 4 (1), 5 (1)6 (1)312.5
S긶06Mugwort pollen373 (3), 4 (2), 6 (2)(0)38.16
I긲04Hazel pollen270 (5), 1 (2)2 (1)10.52
I긲04Hazel pollen362 (1), 3 (4)6 (1)35.43
I긣03Cocksfoot pollen273 (3), 4 (1), 6 (3)(0)22.29
I긣03Cocksfoot pollen363 (3), 5 (1)6 (1)39.39
F긢04Wheat-flour281 (2), 2 (4), 3 (1)0 (1)21.57
F긢04Wheat-flour373 (6)5 (1)312.3
F긢11Buckwheat-flour280 (5), 1 (1), 2 (2)(0)21.39
F긢11Buckwheat-flour373 (3), 4 (2), 5 (1)6 (1)310.10
F긢13Peanut281 (2), 2 (5)3 (1)22.06
F긢13Peanut374 (3), 5 (1), 6 (3)(0)312.90
F긢26Pork370 (4), 1 (2)2 (1)00.25
F긢27Beef370 (3), 1 (1), 2 (3)(0)10.6

The instrument used was from the following company: Polycheck Allergy Diagnostic (Polycheck; Biocheck GmbH, M체nster, Germany); ImmunoCAP250 (Pharmacia Diagnostics AB, Uppsala, Sweden). Bold type is the same class with that of ImmunoCAP..

Abbreviations: S, standard panel; I, inhalant panel; F, food panel..


怨 李

Lim 벑[7]씠 2006뀈 援궡 85媛 寃궗떎쓣 긽쑝濡 븣젅瑜닿린 愿젴 寃궗뱾뿉 븳 꽕臾몄“궗瑜 떎떆븳 寃곌낵 61媛 湲곌뿉꽌 寃궗瑜 떎떆븯怨 엳뿀쑝硫, 遺遺꾩쓽 寃궗떎뱾 寃궗궎듃 궡뿉 룷븿맂 궡遺젙룄愿由щ쭔쓣 떎떆븯怨 엳뿀쑝硫, 젙웾蹂댁젙 嫄곗쓽 떎떆븯吏 븡怨 엳뒗 寃껋쑝濡 議곗궗릺뼱 쇅遺닕젴룄議곗궗쓽 븘슂꽦쓣 吏쟻븯떎. 援궡뿉꽌 鍮꾨줉 留롮 湲곌씠 李몄뿬븳 寃껋 븘땲굹, 援궡뿉꽌 寃泥 寃궗쓽 쇅遺닕젴룄議곗궗궗뾽쓣 떞떦븯뒗 吏꾨떒寃궗쓽븰쉶 븳엫긽寃궗젙룄愿由ы삊쉶뿉꽌뒗 븣젅瑜닿쾺 듅씠 IgE 빆泥댁뿉 븳 쇅遺닕젴룄議곗궗媛 븘吏 뾾뒗 떎젙뿉꽌 蹂 뿰援ъ뿉꽌 떎떆븳 湲곌 媛 룊媛뒗 쇅遺닕젴룄議곗궗瑜 移섑븯뒗 뜲 룄쓣 以 寃껋쑝濡 깮媛곷릺뿀떎. 삤뒪듃由ъ븘 25뀈 寃곌낵 蹂닿퀬뿉꽌룄 遺쟻빀瑜좎 삁쟾遺꽣 李몄뿬븯怨 엳뜕 湲곌뱾씠 떊洹 李몄뿬湲곌뿉 鍮꾪븯뿬 쟻뿀떎怨 蹂닿퀬븯뒗뜲[6], 씠윭븳 寃곌낵뒗 쇅遺닕젴룄議곗궗쓽 吏냽쟻씤 李몄뿬媛 寃궗떎쓽 젙솗꽦쓣 뼢긽븯寃 븯뒗뜲 湲곗뿬븷 닔 엳떎뒗 利앷굅瑜 蹂댁뿬以떎怨 蹂 닔 엳떎.

蹂 뿰援ъ뿉꽌뒗 留ㅽ쉶留덈떎 9湲곌 씠븯쓽 湲곌씠 李몄뿬븯쑝誘濡 븳 紐낆쓽 怨듭뿬옄濡쒕꽣 66 mL쓽 쟾삁쓣 梨꾪삁븯뿬룄 臾대━ 뾾씠 李몄뿬湲곌뿉 룞씪븳 삁泥쓣 젣怨듯븷 닔 엳뿀떎. 洹몃윭굹 援궡湲곌쓣 긽쑝濡 솗븯怨 븳 紐낆쓽 怨듭뿬옄濡쒕꽣 臾쇱쭏쓣 쉷뱷븯뿬빞 븷 寃쎌슦뿉뒗 뿄삁 湲곗뿉 以븯뿬 2二 씠긽쓽 媛꾧꺽쓣 몢怨 삁옣꽦遺꾩콈삁쓣 넻븯뿬 뼸뼱빞 媛뒫븷 寃껋쑝濡 깮媛곷릺뿀떎.

蹂 뿰援ъ뿉꽌 젙떟瑜좎 룊媛湲곗뿉 뵲씪 留ㅼ슦 떎뼇븿쓣 븣 닔 엳뿀떎. 誘멸뎅 CAP 2018뀈 湲곗쓣 뵲瑜 寃쎌슦 3쉶李⑤ 넻븳 李몄뿬湲곌뱾쓽 젙떟瑜좎 57.6%-100.0%濡 쉶李⑤떦 룊洹 젙떟瑜좎 93.0%-97.7%濡 留ㅼ슦 슦닔븯떎. 諛섎㈃ 삤뒪듃由ъ븘쓽 25뀈媛꾩쓽 옄猷뚮 遺꾩꽍븳 Koch Aberer [6]쓽 理쒕퉰룄留뚯쓣 뿀슜踰붿쐞濡 젙븳 湲곗 쟻슜 떆 27.1%-93.2%濡 쉶李⑤떦 룊洹 젙떟瑜좎 66.3%-76.0%濡 궙븘議뚮떎. 씠윭븳 젙떟瑜좎 룞씪븳 湲곗쓣 쟻슜븳 삤뒪듃由ъ븘쓽 25뀈 寃곌낵쓽 룊洹 74.9% 쑀궗븳 寃곌낵瑜 蹂댁떎[6]. 蹂 뿰援ъ뿉꽌 媛 湲곗 쟻슜 떆쓽 젙떟瑜좎쓽 긽愿愿怨꾨 議곗궗븳 寃곌낵 CAP 湲곗怨쇰뒗 Koch Aberer [6]쓽 理쒕퉰룄짹2벑湲 湲곗 쟻슜떆 媛옣 긽愿怨꾩닔媛 넂븯쑝굹, 媛 湲곗뿉 뵲씪 젙떟瑜좎뿉 李⑥씠媛 蹂댁씪 닔 엳쑝誘濡 룊媛옄媛 뼱뼚븳 湲곗쓣 꽑깮븷 寃껋씤吏뿉 빐꽌뒗 異⑸텇엳 怨좊젮븷 븘슂媛 엳寃좊떎. 삁瑜 뱾硫 蹂 뿰援ъ뿉꽌룄 Table 1쓽 A湲곌 CAP 湲곗뿉꽌뒗 3쉶李 紐⑤몢 100% 젙떟瑜좎쓣 蹂댁쓬뿉룄 Koch Aberer [6]쓽 理쒕퉰룄留뚯쓣 뿀슜湲곗쑝濡 젙븳 寃쎌슦뿉뒗 46.2%, 66.7%븘 89.7%쓽 궙 젙떟瑜좎쓣 蹂댁湲 븣臾몄씠떎.

Table 1뿉꽌 蹂대벏씠 CAP 湲곗 쟻슜 떆 뿀슜踰붿쐞瑜 踰쀬뼱궃 湲곌뱾쓽 寃곌낵뒗 遺쟻빀 寃곌낵瑜 蹂댁씤 빆썝쓽 媛쒖닔뿉 긽愿뾾씠 뿀슜踰붿쐞뿉 鍮꾪븯뿬 紐⑤몢 씪愿꾩쟻쑝濡 넂嫄곕굹 샊 궙 뼇긽쓣 蹂댁떎. 遺쟻빀 寃곌낵瑜 蹂댁씤 13湲곌뱾쓽 寃곌낵媛 뿀슜踰붿쐞蹂대떎 넂 寃곌낵뒗 6湲곌, 궙 寃곌낵뒗 7湲곌쑝濡 鍮꾩듂븯湲곕뒗 븯굹, 씠 以 遺쟻빀 寃곌낵媛 25媛쒖 16媛쒕 蹂댁씤 2媛 湲곌쓽 寃곌낵 以 60媛 빆썝 以 class I 씠긽쓣 蹂댁씤 빆썝쓽 媛쒖닔媛 媛 40媛, 43媛쒕 굹궡뿀떎(옄猷 젣떆븯吏 븡쓬). 뵲씪꽌 寃궗 썑 뼇꽦쓣 蹂댁씤 빆썝쓽 媛쒖닔媛 留롮쓣 寃쎌슦뿉뒗 쐞뼇꽦 媛뒫꽦쓣 諛곗젣븯湲 쐞븯뿬 愿젴 湲곌뎄 諛 떆빟愿由 벑쓣 젏寃븷 븘슂媛 엳寃좊떎. 씠 몢 湲곌뱾 씠 씠썑뿉 룊媛뿉 李몄뿬븯吏 븡븘 異붿쟻븷 닔뒗 뾾뿀떎. 씠윭븳 遺쟻빀 寃곌낵뱾 듅젙 쉶李⑥뿉 듅젙 湲곌뿉꽌 삤떟쓣 諛곗텧븯뒗 寃쏀뼢쓣 蹂댁怨, 遺쟻빀쓣 蹂댁씤 紐⑤뱺 湲곌뿉꽌 遺쟻빀쓣 蹂댁씤 뼇긽 떎瑜 湲곌뿉 鍮꾪븯뿬 씪愿꾩쟻쑝濡 넂嫄곕굹 샊 궙嫄곕굹 븯뒗 뼇긽쓣 蹂댁뿬二쇱뿀쑝誘濡 떆빟쓽 듅꽦씠씪湲곕낫떎뒗 寃궗怨쇱젙씠굹 寃궗 愿젴 엳뒗 옣鍮 諛 湲고 슂씤뿉 쓽븳 寃껋엫쓣 븣 닔 엳뿀떎. 뵲씪꽌 젣뭹쓽 臾몄젣씪湲곕낫떎뒗 寃궗떎쓽 닔湲곕쾿씠굹 愿젴 湲곌뎄瑜 젏寃븷 븘슂媛 엳쓣 寃껋쑝濡 蹂댁뿬議뚮떎.

蹂 뿰援ъ뿉꽌 遺쟻빀 寃곌낵뱾 以 뿀슜踰붿쐞뿉 鍮꾪븯뿬 넂 벑湲됱쓣 蹂댁씤 鍮꾩쑉씠 72.1% (49/68) 洹몃━怨 궙 벑湲됱쓣 蹂댁씤 鍮꾩쑉씠 27.9% (19/68)濡 쟾옄媛 뜑 留롮븯떎. 씠윭븳 냼寃ъ 삤뒪듃由ъ븘쓽 25뀈媛꾩쓽 쇅遺닕젴룄룊媛 寃곌낵遺꾩꽍뿉꽌 뼇꽦 寃泥댁쓽 쐞쓬꽦瑜좎 5.2%씤뜲 諛섑븯뿬 쓬꽦 寃泥댁쓽 쐞뼇꽦瑜좎 17.1%濡 뜑 鍮덈쾲븯떎뒗 蹂닿퀬 愿젴씠 엳떎怨 蹂 닔 엳떎[6]. 씠 蹂닿퀬뿉꽌뒗 빆썝援곗뿉 뵲瑜 遺쟻빀 寃곌낵쑉 媛 빆썝援곗뿉 뵲씪 떖옄뒗뜲 쐞뼇꽦瑜좉낵 쐞쓬꽦瑜좎 빟臾 32.8% 17.1%, 룆異 21.7% 3.8%, 쓬떇臾 15.9% 8.8%, 苑껉猷 15.3% 2.8%, 紐곕뱶 11.5% 8.4%, 洹몃━怨 룞臾쇱긽뵾꽭룷 5.2% 4.8%濡 쐞뼇꽦瑜좎씠 뜑 넂븯쑝굹, 솚寃쎈Ъ吏덉쓽 寃쎌슦 쐞쓬꽦씠 7.1%濡 쐞뼇꽦 5.8%蹂대떎 쑀씪븯寃 넂 寃곌낵瑜 蹂댁떎怨 븯떎. 鍮꾨줉 蹂 뿰援ъ뿉꽌뒗 빆썝蹂꾨줈 寃넗븯吏뒗 紐삵븯쑝굹 遺쟻빀쓣 蹂댁씠뒗 빆썝 떇뭹삎뿉 鍮꾪븯뿬 씉엯삎씠 뜑 留롮븯떎.

쑀읇 쇅遺닕젴룄룊媛 1997뀈 蹂닿퀬뿉꽌뒗 24媛쒖쓽 寃곌낵뿉 븯뿬 OMIS 媛믪씠 0 媛옣 씠긽쟻씠硫 <3 留뚯”씠怨 >3씤 寃쎌슦뿉뒗 遺덈웾쑝濡 媛꾩<븯릺 3쉶 뿰냽쑝濡 >3씤 寃쎌슦瑜 吏냽쟻씤 遺덈웾쑝濡 젙쓽븯떎[5]. 蹂 뿰援ъ뿉꽌뒗 몢 湲곌쓽 OMIS 媛믪씠 븳 쉶李⑥뿉 媛곴컖 4젏쓣 蹂댁쑝굹 59媛 빆썝뿉 븳 寃껋씠씪뒗 젏쓣 怨좊젮븯怨 씠瑜 젣쇅븯怨좊뒗 3젏쓣 珥덇낵븯뒗 湲곌씠 뾾뿀湲곗뿉 3쉶 룞븞 蹂 룊媛뿉 李몄뿬븳 湲곌뱾씠 뼇샇븳 寃곌낵瑜 蹂댁쓬쓣 븣 닔 엳뿀떎. 듅엳 OMIS 吏닔뒗 Koch Aberer [6]쓽 遺쟻빀瑜좉낵 넂 긽愿怨꾩닔瑜 蹂댁뿬 遺쟻빀瑜좎쓣 蹂댁뿬二쇰뒗 슚怨쇱쟻씠硫댁꽌 湲곌쓽 늻쟻寃곌낵瑜 醫낇빀븯뿬 李몄뿬湲곌쓽 닔뻾瑜좎쓣 룊媛븷 닔 엳떎뒗 寃껋씠 옣젏쑝濡 뿬寃⑥쭊떎.

쑀읇 쇅遺닕젴룄룊媛 1997뀈 蹂닿퀬뿉꽌 ImmunoCAP UniCap뒗 媛옣 궙 삤瑜섍컪쓣 蹂댁뜕 寃궗踰뺤쑝濡 蹂 뿰援ъ뿉꽌 痢≪젙씠 媛뒫븯뜕 20媛 寃泥댁뿉 븯뿬 痢≪젙븳 寃곌낵 Polycheck뒗 ImmunoCAP 20媛 以 3媛쒖뿉꽌 遺덉씪移 寃곌낵瑜 蹂댁쑝굹 紐⑤몢 븳 벑湲 씠궡떎[5]. 쑀읇 쇅遺닕젴룄룊媛 1997뀈 蹂닿퀬뿉꽌뒗 class 0怨 1 쓬꽦쑝濡 洹 씠긽쓽 벑湲됱 뼇꽦쑝濡 媛꾩<븯뒗뜲, 씠 湲곗뿉 쓽븯硫 遺덉씪移섎 蹂댁씤 3媛쒖쓽 寃泥대뱾룄 紐⑤몢 ImmunoCAP쓽 뼇꽦怨 쓬꽦 뙋젙뿉뒗 씪移섑븯떎[5]. 遺덉씪移섎 蹂댁씤 3醫낅쪟쓽 빆썝뱾쓣 궡렣蹂대㈃ 깉슦뿉 븯뿬 ImmunoCAP (f24)뿉꽌 class 1쓣 蹂댁씤 諛섎㈃ Polycheck뒗 f24/440 (shrimp mix)뒗 異붽 빆썝쓣 媛吏怨 엳뒗뜲 떎닔 湲곌씠 0쑝濡 蹂닿퀬븯뿬 ImmunoCAP 鍮꾪븯뿬 궙寃 痢≪젙릺뿀湲곕뒗 븯굹 紐⑤몢 쓬꽦쑝濡 뙋젙씠 媛뒫븯誘濡, 뼇꽦쑝濡 뙋젙릺뒗 class 2瑜 遺쟻빀 寃곌낵濡 泥섎━븯뒗뜲 臾몄젣媛 뾾뿀떎. 삉븳 삤옃(g03)怨 븙肄(f13) 媛곴컖 몢 寃泥댁뵫 鍮꾧탳媛 媛뒫븯뒗뜲 씠以 븳 寃泥댁뵫뿉꽌留 遺덉씪移섎 蹂댁쑝硫, 씠윭븳 遺덉씪移섎룄 寃궗떎 媛 鍮꾧탳쓽 뿀슜踰붿쐞媛 ImmunoCAP쓽 寃곌낵泥섎읆 紐⑤몢 뼇꽦쑝濡 뙋젙씠 媛뒫븯뿬 쓬꽦쑝濡 뙋젙븯뒗 class 0쑝濡 蹂닿퀬븳 遺쟻빀 湲곌쓣 룊媛븯뒗 뜲 臾대━媛 뾾뿀떎(Table 2).

RIDA allergy screen (R-Biopharm, Darmstadt, Germany), MAST optigen (Hitachi Chemical Diagnostics, Mountain View, CA, USA), Polycheck瑜 ImmunoCAP怨 鍮꾧탳 룊媛븳 援궡 끉臾몄뿉꽌뒗 ImmunoCAP怨쇱쓽 씪移섏쑉씠 뼇꽦 寃泥댁쓽 寃쎌슦 媛곴컖 45.7%, 88.2%, 28.6%쑝硫, 쓬꽦 寃泥댁쓽 寃쎌슦 37.0%, 51.9% 88.9%濡 蹂닿퀬븯떎[8]. 諛섎㈃ MAST CLA allergy system (Hitachi Chemical Diagnostics), Allergy Screen (R-Biopharm, Germany), 洹몃━怨 Polycheck瑜 鍮꾧탳 룊媛븳 떎瑜 援궡 끉臾몄뿉꽌뒗 Polycheck媛 떎瑜 몢 떆빟怨 룞벑븯嫄곕굹 슦닔븯떎怨 蹂닿퀬븯떎[2]. 蹂 뿰援ъ뿉꽌 蹂대벏씠 룞씪븳 寃泥댁뿉 븯뿬 룞씪 떆빟쑝濡 痢≪젙븯뿬룄 湲곌뿉 뵲씪 洹몃━怨 룊媛떆湲곗뿉 뵲씪 떖씪吏 닔 엳쑝誘濡, 븣젅瑜닿쾺 듅씠 IgE 寃궗 떆빟쓽 룊媛 떆뒗 듅젙 lot뿉 븯뿬 븳 湲곌뿉꽌留 痢≪젙븯뒗 寃껊낫떎뒗 븳 湲곌뿉꽌 뿬윭 lot뿉 븯뿬 룊媛븯嫄곕굹 샊 듅젙 lot뿉 븯뿬 룊媛 떆뒗 理쒖냼 뿬윭 湲곌뿉꽌 룊媛븯뒗 寃껋씠 諛붾엺吏곹븷 寃껋쑝濡 뿬寃⑥죱떎.

鍮꾨줉 蹂 뿰援ъ뿉 李몄뿬븳 湲곌 닔媛 留롮 븡븯쑝誘濡 뿬윭 媛吏 룊媛湲곗 쟻슜 떆 李몄뿬湲곌 닔媛 留롮씠 李몄뿬븳 寃쎌슦뿉 鍮꾪븯뿬 룊媛쓽 떊猶곕룄뿉 씪遺 쁺뼢쓣 誘몄튌 닔뒗 엳寃좎쑝굹, 븣젅瑜닿쾺 듅씠 IgE 寃궗쓽 쇅遺닕젴룄룊媛媛 뾾뒗 援궡 쁽떎뿉꽌 Polycheck 떆빟쓣 궗슜븯뒗 寃궗떎쓣 긽쑝濡 泥섏쓬쑝濡 湲곌 媛 룊媛瑜 떎떆븯뿬 蹂닿퀬븯떎뒗 뜲뿉 쓽쓽媛 엳寃좊떎. 삉븳 벑湲됱쓣 룊媛븯뒗 떎뼇븳 湲곗뱾쓣 냼媛쒗븯怨, 씠윭븳 룊媛湲곗뿉 뵲씪꽌 젙떟瑜좎씠 떖씪吏 닔 엳쓬쓣 蹂댁뿬二쇱뼱 룊媛湲곗 꽑깮쓽 以묒슂꽦룄 媛뺤“븯떎. 씠윭븳 냼寃щ뱾 뼢썑 援궡뿉꽌 븣젅瑜닿쾺 듅씠 IgE 빆泥 寃궗쓽 寃궗떎 媛 룊媛瑜 떎떆븯뒗뜲 룄쓣 以 닔 엳쓣 寃껋쑝濡 깮媛곷릺뿀떎.

媛먯궗쓽 湲

씠 뿰援щ뒗 2016뀈룄 닚썝硫붾뵒移 엫긽뿰援щ퉬 吏썝뿉 쓽븯뿬 씠猷⑥뼱議뚮떎(AJIRB-DEV-SUR-16-156).

References
  1. Reddy PM, Nagaya H, Pascual HC, Lee SK, Gupta S, Lauridsen JI, et al. Reappraisal of intracutaneous tests in the diagnosis of reaginic allergy. J Allergy Clin Immunol 1978;61:36-41.
    CrossRef
  2. Jang WR, Nahm CH, Kim JH, Lim DH, Jang TY, Moon YS, et al. Allergen specific IgE measurement with Polycheck Allergy:comparison of three multiple allergen simultaneous tests. Korean J Lab Med 2009;29:465-72.
    Pubmed CrossRef
  3. Jeong S, Jang GC, Cho NJ, Han MS, Kim HS, Sun JY, et al. Analysis of Polycheck Allergy results of the recent two years:comparison with skin prick test and ImmunoCAP. Lab Med Online 2012;2:139-47.
    CrossRef
  4. Kim HS, Tests for allergic disease. Korean Society Laboratory Medicine. Laboratory medicine. 5th ed. Seoul: Panmun Education Co. Ltd, 2014:821.
  5. Fifield R. Diagnostic allergy laboratory external proficiency testing in Europe. Methods 1997;13:33-42.
    Pubmed CrossRef
  6. Koch L, Aberer W. Comparability and quality of IgE-based in vitro allergy diagnosis:25 years of external quality assessment. Wien Klin Wochenschr 2014;126:634-41.
    Pubmed CrossRef
  7. Lim HS, Kim HS, Oh H. Current status of serum allergen tests in Korea. Korean J Lab Med 2008;28:124-9.
    Pubmed CrossRef
  8. Han M, Shin S, Park H, Park KU, Park MH, Song EY. Comparison of three multiple allergen simultaneous tests:RIDA allergy screen, MAST optigen, and polycheck allergy. Biomed Res Int 2013 2013:340513.
    Pubmed KoreaMed CrossRef