J Lab Med Qual Assur 2015; 37(3): 124-133
Published online September 30, 2015
Copyright © Korean Association of External Quality Assessment Service.
Young Joo Cha1, Jae Hoon Bae1, Quehn Park1, and Seok Lae Chae2, as Immunoserology Subcommittee, The Korean Association of External Quality Assessment Service
1Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul; 2Department of Laboratory Medicine, Dongguk University Ilsan Hospital, Dongkuk University College of Medicine, Goyang, Korea
Correspondence to:Young Joo Cha
Department of Laboratory Medicine, Chung-Ang University College of Medicine, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Korea
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
As Immunoserology Subcommittee of the Korean Association of External Quality Assessment Service, we organized two trials on the external quality assessment of viral markers and serological tests for syphilis (STS) in 2014. For this purpose, we delivered three kinds of pooled sera specimens for external proficiency testing to 1,060 and 1,064 institutions for the first and second trials, respectively. Pooled sera were checked for their homogeneity and stability by using more than three other methods between the day of their manufacture and 3 days after despatching. The numbers of participating laboratories were 1,053 (99.3%) and 1,046 (99.3%) in the first and second trials, respectively. The most commonly tested items were hepatitis B surface antigen, followed by antibody to hepatitis B surface antigen, anti-human immunodeficiency virus, anti-hepatitis C virus, STS, and anti-hepatitis B core. The most frequently used methods for detecting viral markers were the chemiluminescence immunoassay (CLIA) and the electrochemiluminescence immunoassay, which generated few false positive results. In contrast, false negative results were frequently found through the immunochromatography assay, the use of which for detecting viral markers has been steadily increasing in recent years. Furthermore, the use of turbidoimmunoassay and CLIA, which are new tests recently introduced for the measurement of non-treponemal and treponemal antibodies, is also increasing.
(J Lab Med Qual Assur 2015;37:124-133)
Keywords: External quality assessment, Hepatitis B, Hepatitis C, HIV, Immunoassay, Laboratory proficiency testing, Serology, Syphilis
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