Distribution of the methods and probe types used for the FISH survey and the results for the participants from 2016
| Specimen | Intended responses | Manufacturer | Probe type | No. of laboratories | No. of participants with an acceptable response (n/N |
|---|---|---|---|---|---|
| GF-16-01 | Consistent with trisomy 21 | Vysis | Single color | 4 | 18/19 (94.7) |
| Dual color | 3 | ||||
| Dual fusion | 2 | ||||
| Extra signal | 1 | ||||
| CytoCell | Dual color | 3 | |||
| Single color | 2 | ||||
| MetaSystems | Dual color | 2 | |||
| Dual fusion | 1 | ||||
| Others | Dual color | 1 | |||
| GF-16-02 | Abnormal with a gain of MLL | Vysis | Break-apart | 14 | 25/25 (100.0) |
| Dual color | 1 | ||||
| CytoCell | Break-apart | 2 | |||
| Dual color | 1 | ||||
| MetaSystems | Break-apart | 5 | |||
| Dual color | 1 | ||||
| Dual fusion | 1 | ||||
| GF-16-03 | Abnormal with a deletion of 13q14.3 | Vysis | Single color | 3 | 19/19 (100.0) |
| Dual color | 3 | ||||
| CytoCell | Dual color | 6 | |||
| MetaSystems | Dual color | 6 | |||
| Triple color | 1 | ||||
| GF-16-04 | Abnormal with a gain of IGH | Vysis | Break-apart | 6 | 16/16 (100.0) |
| Dual color | 1 | ||||
| CytoCell | Break-apart | 2 | |||
| Dual fusion | 1 | ||||
| MetaSystems | Break-apart | 6 | |||
| GF-16-05 | Abnormal with a loss of TP53 | Vysis | Dual color | 5 | 20/20 (100.0) |
| CytoCell | Dual color | 5 | |||
| MetaSystems | Dual color | 10 | |||
| GF-16-06 | Mosaic X | Vysis | Dual color | 9 | 18/18 (100.0) |
| Triple color | 1 | ||||
| CytoCell | Dual color | 5 | |||
| MetaSystems | Dual color | 2 | |||
| Dual fusion | 1 |
The following FISH methods were used: Vysis (Downers Grove, IL, USA), CytoCell (Cambridge, UK), and MetaSystems (Altlussheim, Germany).
Abbreviation: FISH, fluorescent in situ hybridization.
n/N indicates the acceptable number/total number of participants.