Table. 2.

Changes in the levels of anti-dsDNA, anti-Tg, and anti-TPO in the pooled sera according to the storage condition and duration

Analyte Storage status Baseline 1 week 2 weeks 1 month 3 months 6 months






Mean±SD CV (%) Mean±SD CV (%) Mean±SD CV (%) Mean±SD CV (%) Mean±SD CV (%) Mean±SD CV (%)
Anti-dsDNA (IU/mL)
N –20℃ <10 NA <10 NA <10 NA <10 NA <10 NA <10 NA
4℃ <10 NA <10 NA <10 NA <10 NA <10 NA <10 NA
RT <10 NA <10 NA <10 NA <10 NA <10 NA <10 NA
P –20℃ 80.9±2.3 2.8 90.5±4.1 4.5 99±4.55 4.6 100±4.7 4.7 102.4±4.51 4.4 114.1±12.9 11.3
4℃ 80.9±2.3 2.8 91.4±3.7 4.1 96.3±7.32 7.6 89.9±4.67 5.2 99.3±5.66 5.7 105±7.77 7.4
RT 80.9±2.3 2.8 89.7±5.2 5.8 90.3±8.49 9.4 834±66.7 8 86.6±7.1 8.2 96.4±16.7 17.3
Anti-Tg (IU/mL)
L –20℃ 126.4±6.1 4.8 112.4±7.5 6.7 107.3±6.55 6.1 107.6±7.96 7.4 81.1±7.7 9.5 61.6±7.02 11.4
4℃ 126.4±6.1 4.8 112.5±5.7 5.1 102.5±5.43 5.3 97±6.6 6.8 67±5.03 7.5 51.2±4.76 9.3
RT 126.4±6.1 4.8 103.7±7.6 7.3 105.7±7.4 7 91±7.28 8 47.5±4.18 13.4 43.3±3.81 8.8
H –20℃ 2,366.4±146.7 6.2 2,352.4±171.7 7.3 2,277.3±118 5.2 2,237.6±192 8.6 2,181.1±109 5 2,090.6±322 15.4
4℃ 2,366.4±146.7 6.2 2,302.5±227.9 9.9 2,242.5±114 5.1 2,197±105 4.8 2,207±99.3 4.5 2,001.2±304 15.2
RT 2,366.4±146.7 6.2 2,293.7±364.7 15.9 2,225.7±55.6 2.5 2,131±85.2 4 2,087.5±148 7.1 1,863.3±231 12.4
Anti-TPO (IU/mL)
L –20℃ 36.8±2.0 5.3 35.7±2.7 7.6 37.2±3.01 8.1 36.6±2.78 7.6 34±2.52 7.4 30.9±1.82 5.9
4℃ 36.8±2.0 5.3 31.1±2.1 6.7 29.9±1.76 5.9 32.2±2.61 8.1 27.3±1.86 6.8 25.7±1.82 7.1
RT 36.8±2.0 5.3 30.7±1.9 6.1 24.7±2 8.1 27±1.49 5.5 26±2 7.7 21.2±1.7 8
H –20℃ 412.8±22.7 5.5 385.7±39.3 10.2 387.2±21.7 5.6 376.6±25.6 6.8 362.6±23.9 6.6 357.9±39.4 11
4℃ 412.8±22.7 5.5 379.1±19.0 5 369.9±24.8 6.7 366.2±27.5 7.5 357.3±27.2 7.6 342.7±37 10.8
RT 412.8±22.7 5.5 374.7±25.1 6.7 364.7±34.6 9.5 357±40.3 11.3 346.1±30.8 8.9 328.2±20.3 6.2

Abbreviations: anti-dsDNA, anti-double stranded DNA immunoglobulin G antibody; anti-Tg, anti-thyroglobulin antibody; anti-TPO, anti-thyroperoxidase antibody; SD, standard deviation; CV, coefficient of variation; N, negative; P, positive; L, low; H, high; RT, room temperature (20℃–24℃); NA, not available.

Lab Med Qual Assur 2021;43:72~79 https://doi.org/10.15263/jlmqa.2021.43.2.72
© Lab Med Qual Assur