Laboratory Medicine and Quality Assurance

Table. 1.

PNH clones (%) of RBCs and neutrophils of the patients and controls at 5 different storage conditions

Variable Fresh EDTA WB EDTA WB (4℃, 72 hr) EDTA WB (RT, 72 hr) 10% PM70 WB (RT, 72 hr) Cyto-chex WB (RT, 72 hr) TransFix WB (RT, 72 hr)
Patients (N=20)
RBCs 56.05 (0.09–99.46) 57.08 (0.07–99.33) 53.18 (0.07–90.38) 56.52 (0.08–99.29) 58.28 (0.08–99.38) 56.94 (0.07–99.39)
P-value 0.067 <0.001 0.062 0.135 0.940
WBCs 89.70 (0.14–99.71) 88.68 (0.14–98.44) 86.25 (0.10–95.11) 88.55 (0.15–99.54) 88.37 (0.18–99.17) 88.84 (0.19–97.44)
P-value 0.079 <0.001 0.108 0.058 0.062
Controls (N=15)
RBCs 0.00 (0.00–0.70) 0.00 (0.00–0.80) 0.00 (0.00–0.65) 0.00 (0.00–0.79) 0.00 (0.00–0.77) 0.00 (0.00–0.81)
P-value 0.151 0.078 0.194 0.151 0.151
Neutrophils 0.05 (0.03–0.32) 0.05 (0.03–0.42) 0.04 (0.00–0.25) 0.05 (0.03–0.46) 0.06 (0.00–0.40) 0.07 (0.03–0.49)
P-value 0.249 0.022 0.117 0.330 0.131

Values are presented as median % (range %).

Abbreviations: PNH, paroxysmal nocturnal hemoglobinuria; RBCs, red blood cells; EDTA, ethylenediaminetetraacetic acid; WB, whole blood; RT, room temperature; WBCs, white blood cells.

Lab Med Qual Assur 2021;43:143~151 https://doi.org/10.15263/jlmqa.2021.43.3.143
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