Table. 10.

Number of participating laboratories according to the technique for ABO antibody titration

Trial Technique No. Acceptable range (%) Titer

4 8 16 32 64 128 256 512 1024
Anti-A
1st (BBS-20-03) Tube-IS 38 97.4 2 20 15 1
Tube-RT 22 100.0 6 14 2
Tube-AHG 21 100.0 4 13 4
Tube-AHG+DTT 5 NA 1 3 1
CAT-RT 15 100.0 15
CAT-AHG 33 100.0 1 16 16
CAT-AHG+DTT 5 NA 2 3
Others 1 NA 1
Total 140 NA 2 27 50 34 26 1
2nd (BBS-20-06) Tube-IS 35 94.3 2 18 13 2
Tube-RT 25 100.0 6 17 2
Tube-AHG 21 100.0 5 16
Tube-AHG+DTT 6 NA 2 2 1
CAT-RT 15 100.0 7 8
CAT-AHG 35 100.0 5 24 6
CAT-AHG+DTT 5 NA 1 2 2
Others 1 NA 1
Total 142 NA 2 34 51 47 8
Anti-B
1st (BBS-20-03) Tube-IS 38 97.4 6 22 9 1
Tube-RT 22 95.5 1 10 11
Tube-AHG 21 95.2 1 7 12 1
Tube-AHG+DTT 5 NA 1 1 2 1
CAT-RT 15 100.0 13 2
CAT-AHG 33 100.0 10 17 6
CAT-AHG+DTT 5 NA 4 1
Others 1 NA 1
Total 140 NA 7 47 40 35 10 1
2nd (BBS-20-06) Tube-IS 35 97.1 12 17 5 1
Tube-RT 25 100.0 1 12 12
Tube-AHG 21 100.0 1 13 7
Tube-AHG+DTT 5 NA 2 2 1
CAT-RT 15 100.0 1 9 5
CAT-AHG 35 97.1 1 11 18 5
CAT-AHG+DTT 5 NA 1 1 2 1
Others 1 NA 1
Total 142 NA 15 40 27 26 28 6

Acceptable results are indicated in bold.

Abbreviations: IS, immediate spin; RT, room temperature incubation; AHG, indirect anti-human globulin test; DTT, dithiothreitol; CAT, column agglutination technology; NA, not applicable.

Lab Med Qual Assur 2021;43:167~175 https://doi.org/10.15263/jlmqa.2021.43.4.167
© Lab Med Qual Assur