Table. 13.

Number of participants according to the technique of ABO antibody titration

Trial Technique No. Acceptable range (%) Titer

4 8 16 32 64 128 256 512 1024
Anti-A
1st (BBS-21-03) Tube-IS 32 93.8 1 9 20 1 1
Tube-RT 23 95.7 1 15 6 1
Tube-AHG 20 100.0 2 14 4
Tube-AHG+DTT 5 NA 3 1 1
CAT-RT 16 93.8 6 7 2 1
CAT-AHG 35 97.1 1 15 17 2
CAT-AHG+DTT 5 NA 2 3
Others 1 NA 1
Total 137 NA 1 17 42 15 34 26 2
2nd (BBS-21-06) Tube-IS 32 93.8 2 6 18 6
Tube-RT 21 95.2 1 8 12
Tube-AHG 20 95.0 2 12 5 1
Tube-AHG+DTT 5 NA 3 1 1
CAT-RT 18 100.0 6 11 1
CAT-AHG 41 100.0 8 22 11
CAT-AHG+DTT 5 NA 1 3 1
Total 142 NA 2 7 32 34 23 31 13
Anti-B
1st (BBS-21-03) Tube-IS 32 93.8 1 6 22 2 1
Tube-RT 23 95.7 17 5 1
Tube-AHG 20 100.0 2 13 5
Tube-AHG+DTT 5 NA 3 2
CAT-RT 16 93.8 3 10 2 1
CAT-AHG 35 97.1 1 13 19 2
CAT-AHG+DTT 5 NA 2 3
Others 1 NA 1
Total 137 NA 1 10 49 15 32 27 3
2nd (BBS-21-06) Tube-IS 32 87.5 4 11 16 1
Tube-RT 21 100.0 1 14 6
Tube-AHG 20 100.0 4 15 1
Tube-AHG+DTT 5 NA 1 3 1
CAT-RT 18 100.0 1 12 5
CAT-AHG 41 92.7 8 21 9 3
CAT-AHG+DTT 5 NA 1 4
Total 142 NA 4 13 43 28 41 10 3

Acceptable results are indicated in bold.

Abbreviations: IS, immediate spin; RT, room temperature incubation; AHG, indirect anti-human globulin test; DTT, dithiothreitol; CAT, column agglutination technology; NA, not applicable.

Lab Med Qual Assur 2022;44:10~20 https://doi.org/10.15263/jlmqa.2022.44.1.10
© Lab Med Qual Assur