Analytical instrument conditions for ultra-high performance liquid chromatography
| Variable | Values/features |
|---|---|
| Mobile phase A | 0.1% formic acid in water |
| Mobile phase B | 100% acetonitrile |
| Gradient | |
| 0.01 min | A (85%), B (15%) |
| 1.00 min | A (85%), B (15%) |
| 3.50 min | A (30%), B (70%) |
| 3.60 min | A (10%), B (90%) |
| 4.50 min | A (10%), B (90%) |
| 4.60 min | A (85%), B (15%) |
| 7.00 min | A (85%), B (15%) |
| Washing solvent | 50% MeOH |
| Autosampler temperature | 10℃ |
| Injection volume | 5 μL |
| Run time | 7 min |
| Flow rate | 0.4 mL/min |