Laboratory Medicine and Quality Assurance

Table. 17.

Methods and results of HLA crossmatching in 2021

Trials Specimen Methods Total no. of labs Qualitative results (no. of labs) Semi-quantitative results (no. of labs) Intended response
Negative Positive NA Negative 1:1 1:2 1:4 1:8 1:16 1:32 >1:32
1st IX-21-01 + IX-21-03 T-CDC-Direct 44 44 0 44 Negative
T-CDC-AHG 46 46 0 46 Negative
B-CDC-Direct 23 20 3 20 3 Negative
T-FCM 33 33 0 Negative
B-FCM 26 10 16 Code 505
IX-21-01 + IX-21-04 T-CDC-Direct 44 44 0 44 Negative
T-CDC-AHG 46 46 0 46 Negative
B-CDC-Direct 23 16 7 16 3 1 2 1 Code 505
T-FCM 33 33 0 Negative
B-FCM 26 3 23 Positive
IX-21-02 + IX-21-03 T-CDC-Direct 44 42 2 42 1 1 Negative
T-CDC-AHG 46 44 2 44 1 1 Negative
B-CDC-Direct 23 1 22 1 1 1 11 9 Positive
T-FCM 33 33 0 Negative
B-FCM 26 1 25 Positive
IX-21-02 + IX-21-04 T-CDC-Direct 44 42 2 42 1 1 Negative
T-CDC-AHG 46 44 2 44 1 1 Negative
B-CDC-Direct 23 17 6 17 3 2 1 Code 505
T-FCM 33 21 12 Code 505
B-FCM 26 1 25 Positive
2nd IX-21-05 + IX-21-07 T-CDC-Direct 44 37 7 37 1 2 1 3 Code 505
T-CDC-AHG 46 27 19 27 5 4 2 1 4 3 Code 505
B-CDC-Direct 24 0 24 1 1 2 6 11 3 Positive
T-FCM 33 3 30 Positive
B-FCM 26 0 26 Positive
IX-21-05 + IX-21-08 T-CDC-Direct 44 43 1 43 1 Negative
T-CDC-AHG 46 44 2 44 1 1 Negative
B-CDC-Direct 24 1 23 1 1 1 2 4 11 4 Positive
T-FCM 33 16 17 Code 505
B-FCM 26 0 26 Positive
IX-21-06 + IX-21-07 T-CDC-Direct 44 5 39 5 5 5 13 9 2 4 1 Positive
T-CDC-AHG 46 0 46 5 6 9 6 16 4 Positive
B-CDC-Direct 24 0 24 1 2 4 3 12 2 Positive
T-FCM 33 0 33 Positive
B-FCM 26 0 26 Positive
IX-21-06 + IX-21-08 T-CDC-Direct 44 42 2 42 1 1 Negative
T-CDC-AHG 46 41 5 41 1 1 2 1 Code 505
B-CDC-Direct 24 2 22 2 1 1 1 2 3 10 4 Positive
T-FCM 33 19 14 Code 505
B-FCM 26 0 26 Positive

Bold letter indicates the results which are not compliant with the intended responses.

Abbreviations: HLA, human leukocyte antigen; NA, not available; CDC-Direct, Center for Disease Control and Prevention-Direct; AHG, anti-human globulin; FCM, flow cytometry.

Lab Med Qual Assur 2024;46:1~37 https://doi.org/10.15263/jlmqa.2024.46.1.1
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