HLA typing methods and frequently used kits
Methods/kits | 2022 | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1st | 2nd | ||||||||||||
HLA-A | HLA-B | HLA-C | HLA-DR | HLA-DQ | HLA-DP | HLA-A | HLA-B | HLA-C | HLA-DR | HLA-DQ | HLA-DP | ||
PCR-SSP | 23 | 23 | 13 | 23 | 13 | 0 | 24 | 24 | 14 | 24 | 14 | 0 | |
Micro SSP Generic Trays, One Lambda | |||||||||||||
HLA PCR/SSP Kit, Biosewoom | |||||||||||||
PCR-SSOP | 28 | 28 | 13 | 28 | 23 | 1 | 27 | 27 | 12 | 27 | 22 | 1 | |
LABType SSO, One Lambda | |||||||||||||
INNO LiPA HLA kit, Fujirebio | |||||||||||||
SBT | 16 | 16 | 16 | 16 | 9 | 1 | 15 | 15 | 14 | 15 | 9 | 1 | |
SeCore SBT kit, One Lambda | |||||||||||||
Allele SEQR HLA, GenDx | |||||||||||||
AVITA cross HLA SBT kit, BIOWITHUS | |||||||||||||
AVITA plus HLA SBT kit, BIOWITHUS | |||||||||||||
AVITA HLA SBT kit, BIOWITHUS | |||||||||||||
NGS | 12 | 12 | 11 | 12 | 10 | 6 | 13 | 13 | 12 | 13 | 12 | 8 | |
NGSgo HLA-NGS (6 loci), GenDx | |||||||||||||
AllType NGS (11 loci), One Lambda | |||||||||||||
Others | 4 | 4 | 4 | 4 | 4 | 1 | 2 | 2 | 2 | 2 | 1 | 1 | |
Total | 83 | 83 | 57 | 83 | 59 | 9 | 81 | 81 | 54 | 81 | 58 | 11 |
The use of LIFECODES HLA SSO typing kit was stopped since 2021. The instruments were from the following companies: One Lambda (Canoga Park, CA, USA), Biosewoom (Seoul, Korea), Fujirebio Inc. (Tokyo, Japan), GenDx (Utrecht, The Netherlands), and Biowithus Inc. (Seoul, Korea).
Abbreviations: HLA, human leukocyte antigen; PCR, polymerase chain reaction; SSP, sequence specific primers; SSOP, sequence specific oligonucleotide probe; SBT, sequence-based typing; NGS, next generation sequence.