Conversion factors (IU/copies) derived from various BK virus quantification PCR procedures
Reagent | Real-time PCR machines | Nucleic acid extraction instruments | Extraction kits | Conversion factor | References | ||
---|---|---|---|---|---|---|---|
Plasma | Urine | ||||||
Real-Q BK Virus | CFX96 DX | Real-Prep | Real-Prep | 0.48 | 0.19 | This study | |
NS | QIAamp DSP virus spin kit | 0.37 | NS | Company-provided | |||
kPCR PLX BKV DNA assay | Versant kPCR Molecular systems AD | NS | Versant sample preparation 1.2 reagents | 1.67 | 0.33 | [8] |
Abbreviations: PCR, polymerase chain reaction; NS, not specified.