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pISSN 2950-9114 eISSN 2950-9122
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Brief Communication

Lab Med Qual Assur 2021; 43(4): 214-216

Published online December 31, 2021

https://doi.org/10.15263/jlmqa.2021.43.4.214

Copyright © Korean Association of External Quality Assessment Service.

Comparison Study of Molecular Diagnostic Reagents for COVID-19 Pooling Test

Dong Hee Seo1 , Geun-ju Son2 , and Jeong-woo Kang2

1LabGenomics Clinical Laboratories and 2Division of Molecular Genetics Examination, LabGenomics Co., Seongnam, Korea

Correspondence to:Dong Hee Seo
LabGenomics Clinical Laboratories, LabGenomics Co., B-6F, 700 Daewangpangyo-ro, Bundang-gu, Seongnam 13488, Korea
Tel +82-31-628-0730
E-mail seo2023@labgenomics.com

Received: July 6, 2021; Revised: September 7, 2021; Accepted: September 8, 2021

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In Korea, real-time reverse transcription-polymerase chain reaction (PCR) is performed on individual or pooled samples to diagnosis coronavirus disease 2019 (COVID-19). New developed LabGun COVID-19 ExoFast RT-PCR Kit (LabGenomics, Korea) was compared to PowerCheck SARS-CoV-2 Real-time PCR Kit (KogeneBiotech, Korea) for its properness about COVID-19 pooling test. LabGun kit showed 98.75% concordance for positive samples and 100% concordance for negative samples tested by PowerCheck kit. Agarose gel electrophoresis study revealed similar PCR products band intensities for two testing kits. In conclusion, LabGun kit could be used for COVID-19 pooling test.

Keywords: COVID-19, RT-PCR testing, Pooling test

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