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pISSN 2384-2458 eISSN 2288-7261
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Original Article

J Lab Med Qual Assur 2016; 38(3): 151-158

Published online September 30, 2016


Copyright © Korean Association of External Quality Assessment Service.

Evaluation of the Effectiveness of NK Vue Gold Kit in Patients with Chronic Hepatitis B

Young Ae Lim1, Soon Sun Kim2, Sung Won Cho2, and Jae Youn Cheong2

Departments of 1Laboratory Medicine and 2Gastroenterology, Ajou University School of Medicine, Suwon, Korea

Correspondence to:Young Ae Lim
Department of Laboratory Medicine, Ajou University Hospital, Ajou University School of Medicine, 164 World cup-ro, Yeongtong-gu, Suwon 16499, Korea
Tel: +82-31-219-5786 Fax: +82-31-219-5778 E-mail: limyoung@ajou.ac.kr

Received: April 19, 2016; Revised: June 23, 2016; Accepted: July 1, 2016

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background: The associations between hepatitis B virus (HBV) infection and natural killer cell activity (NKA) have been studied. However, there have been no studies on detection of NKA in HBV patients using NK Vue Gold kit (ATGen Co., Korea), which has been developed in Korea and can easily detect NKA in whole blood.
Methods: A patient group of 40 patients diagnosed with hepatitis B antigen-positive chronic hepatitis B and a control group of 40 people eligible for blood donation were selected for this study. Whole blood (1 mL) was collected in special tubes for analysis with NK Vue Gold kit. The concentration of interferon-γ (IFN-γ) was measured and considered to represent NKA.
Results: There was no significant difference in the median (value, range) of concentration of IFN-γ between the patient (2,000, 89?2,000 pg/mL) and control groups (1,786, 333?2,000 pg/mL). The concentration of IFN-γ was inversely correlated with leukocyte count in both patient (r =?0.390, P=0.022) and control (r =?0.339, P=0.032) groups. The concentration of IFN-γ in the patient group was inversely correlated with concentrations of alanine aminotransferase (r =?0.363, P=0.021), aspartate aminotransferase (r =?0.336, P=0.037), and bilirubin (r =?0.376, P=0.020).
Conclusions: There was no significant difference in NKA between the patient and control group. Caution should be practiced when interpreting results of NKA, because leukocyte count can affect concentration of IFN-γ.

Keywords: Natural killer cells, Hepatitis B virus, Interferon-gamma


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