Cellular Hemoglobin Is a Useful Alternative Parameter when Lipemia Interferes with Hemoglobin Measurement

Byong Ho Choi; Sung Ran Cho; Il Joong Park

doi:10.15263/jlmqa.2017.39.1.9

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Original Article

J Lab Med Qual Assur 2017; 39(1): 9-15

Published online March 31, 2017

https://doi.org/10.15263/jlmqa.2017.39.1.9

Cellular Hemoglobin Is a Useful Alternative Parameter when Lipemia Interferes with Hemoglobin Measurement

Byong Ho Choi¹, Sung Ran Cho², Il Joong Park²

¹Department of Laboratory Medicine, Ajou University Hospital, Suwon, Korea,
²Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Korea

Correspondence to:Il Joong Park Department of Laboratory Medicine, Ajou University School of Medicine, 164 World cup-ro, Yeongtong-gu, Suwon 16499, Korea Tel: +82-31-219-5792 Fax: +82-31-219-5778 E-mail: pij0304@daum.net
박일중 우)16499 수원시 영통구 월드컵로 164, 아주대학교 의과대학 진단검사의학교실 Tel: 031)219-5792, Fax: 031)219-5778, E-mail: pij0304@daum.net

Received: November 8, 2016; Revised: January 2, 2017; Accepted: January 23, 2017

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

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Abstract

Background:

Saline replacement is a difficult and time-consuming procedure employed to measure hemoglobin (Hb) levels when lipaemia interferes with the accurate determination of Hb content. As an alternative method, we tested the reliability of cellular Hb (cHb) measurement.

Methods:

Forty-eight lipemic blood samples were analysed with the LH780 (or DxH 800; Beckman Coulter Inc., USA) and ADVIA 2120i (Siemens Healthcare Diagnostics, USA) instruments. We compared the Hb measurements obtained following saline replacement (srHb) with the cHb measurement and with the value of one-third of the hematocrit (1/3Hct).

Results:

The bias estimate outcomes of cHb with srHb were found to be acceptable at all medical decision points. The average difference between the value of 1/3Hct and initial Hb, srHb, and cHb were 19.7%±3.3%, 2.3%±1.6%, and -0.1%±1.1%, respectively.