• Sitemap
  • Contact Us

pISSN 2950-9114 eISSN 2950-9122
Article View

Report On Proficiency Testing

Lab Med Qual Assur 2024; 46(1): 1-37

Published online March 31, 2024

https://doi.org/10.15263/jlmqa.2024.46.1.1

Copyright © Korean Association of External Quality Assessment Service.

Report of Korean Association of External Quality Assessment Service on Histocompatibility Testing (2019–2022)

Eun-Suk Kang* and Jong Kwon Lee*

Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

Correspondence to:Eun-Suk Kang
Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Korea
Tel +82-2-3410-2703
E-mail eskang@skku.edu

*These authors contributed equally to this work as the first author.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Here, we report the results of the histocompatibility proficiency testing performed by the Korean Association of External Quality Assessment Service from 2019 to 2022. The HLA-B51 and HLA-DPB1 typing programs were adopted in 2020 and 2021, respectively. The HLA-B*58:01 typing program was commenced from the second trial in 2022 as a demonstration program. The directly prepared proficiency testing specimens of whole blood, sera, and mononuclear cell suspensions were distributed to participants biannually. The enrollment of participants was expanded for high-resolution human leukocyte antigen (HLA) typing, B cell flow cytometry crossmatching (B-FCMXM), and single antigen bead antibody identification (SABID) tests. The phase and method combination for HLA crossmatching demonstrated variability in terms of laboratory results, and the grading criteria were revised in 2020. The response rate was 100% except that for B-FCMXM. The accuracy rates were generally very high; however, frequent input or nomenclature errors for HLA typing results and frequent non-consensus (code 505) and wide distribution of semiquantitative Center for Disease Control and Prevention results between laboratories were noted. The statistical analysis of reported HLA antibodies rendered the determination of unacceptable responses easier than earlier methods; however, the absence of consensus criteria meant that the responses were not graded yet. Continuous efforts to improve the proficiency programs and the education for standardization between laboratories are mandatory.

Keywords: Histocompatibility, Laboratory proficiency testing, Korean Association of External Quality Assessment Service

Supplementary File


Share this article on :

Stats or metrics